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Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomati...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551749/ https://www.ncbi.nlm.nih.gov/pubmed/26327807 http://dx.doi.org/10.7150/ijbs.11946 |
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author | Zavan, Barbara De Francesco, Francesco D'Andrea, Francesco Ferroni, Letizia Gardin, Chiara Salzillo, Rosa Nicoletti, Gianfranco Ferraro, Giuseppe A. |
author_facet | Zavan, Barbara De Francesco, Francesco D'Andrea, Francesco Ferroni, Letizia Gardin, Chiara Salzillo, Rosa Nicoletti, Gianfranco Ferraro, Giuseppe A. |
author_sort | Zavan, Barbara |
collection | PubMed |
description | Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomatic, they can cause symptoms due to nerve or vessel compression. Microscopically they appear as fibrous connective tissue stroma with embedded adipocytes, and absence of inflammation. Up to now no characterisation of stem cell population present in this tissue has been performed. Methods: Cytofluorimetric, biological and molecular biology analyses have been performed in order to test superficial cell markers and gene expression profile related to stemness and apoptotic activity of cells present in lipoma tissues compared to those of adipose tissue's cells. Results: Our results confirmed that CD34(+) cells in lipoma were present around small adipocytes, showing several altered biological activity such as proliferation, apoptotis and stemness. Conclusions: The data emerging from the comparison of the lipoma cells and normal adipose tissue, suggests the presence of cell precursors involved in the development of the lipoma. This hypothesis requires further investigation and may indicate new thresholds in the study of benign tumour pathogenesis. |
format | Online Article Text |
id | pubmed-4551749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-45517492015-08-31 Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells Zavan, Barbara De Francesco, Francesco D'Andrea, Francesco Ferroni, Letizia Gardin, Chiara Salzillo, Rosa Nicoletti, Gianfranco Ferraro, Giuseppe A. Int J Biol Sci Research Paper Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomatic, they can cause symptoms due to nerve or vessel compression. Microscopically they appear as fibrous connective tissue stroma with embedded adipocytes, and absence of inflammation. Up to now no characterisation of stem cell population present in this tissue has been performed. Methods: Cytofluorimetric, biological and molecular biology analyses have been performed in order to test superficial cell markers and gene expression profile related to stemness and apoptotic activity of cells present in lipoma tissues compared to those of adipose tissue's cells. Results: Our results confirmed that CD34(+) cells in lipoma were present around small adipocytes, showing several altered biological activity such as proliferation, apoptotis and stemness. Conclusions: The data emerging from the comparison of the lipoma cells and normal adipose tissue, suggests the presence of cell precursors involved in the development of the lipoma. This hypothesis requires further investigation and may indicate new thresholds in the study of benign tumour pathogenesis. Ivyspring International Publisher 2015-07-18 /pmc/articles/PMC4551749/ /pubmed/26327807 http://dx.doi.org/10.7150/ijbs.11946 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Zavan, Barbara De Francesco, Francesco D'Andrea, Francesco Ferroni, Letizia Gardin, Chiara Salzillo, Rosa Nicoletti, Gianfranco Ferraro, Giuseppe A. Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title | Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title_full | Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title_fullStr | Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title_full_unstemmed | Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title_short | Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells |
title_sort | persistence of cd34 stem marker in human lipoma: searching for cancer stem cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551749/ https://www.ncbi.nlm.nih.gov/pubmed/26327807 http://dx.doi.org/10.7150/ijbs.11946 |
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