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Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells

Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomati...

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Autores principales: Zavan, Barbara, De Francesco, Francesco, D'Andrea, Francesco, Ferroni, Letizia, Gardin, Chiara, Salzillo, Rosa, Nicoletti, Gianfranco, Ferraro, Giuseppe A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551749/
https://www.ncbi.nlm.nih.gov/pubmed/26327807
http://dx.doi.org/10.7150/ijbs.11946
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author Zavan, Barbara
De Francesco, Francesco
D'Andrea, Francesco
Ferroni, Letizia
Gardin, Chiara
Salzillo, Rosa
Nicoletti, Gianfranco
Ferraro, Giuseppe A.
author_facet Zavan, Barbara
De Francesco, Francesco
D'Andrea, Francesco
Ferroni, Letizia
Gardin, Chiara
Salzillo, Rosa
Nicoletti, Gianfranco
Ferraro, Giuseppe A.
author_sort Zavan, Barbara
collection PubMed
description Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomatic, they can cause symptoms due to nerve or vessel compression. Microscopically they appear as fibrous connective tissue stroma with embedded adipocytes, and absence of inflammation. Up to now no characterisation of stem cell population present in this tissue has been performed. Methods: Cytofluorimetric, biological and molecular biology analyses have been performed in order to test superficial cell markers and gene expression profile related to stemness and apoptotic activity of cells present in lipoma tissues compared to those of adipose tissue's cells. Results: Our results confirmed that CD34(+) cells in lipoma were present around small adipocytes, showing several altered biological activity such as proliferation, apoptotis and stemness. Conclusions: The data emerging from the comparison of the lipoma cells and normal adipose tissue, suggests the presence of cell precursors involved in the development of the lipoma. This hypothesis requires further investigation and may indicate new thresholds in the study of benign tumour pathogenesis.
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spelling pubmed-45517492015-08-31 Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells Zavan, Barbara De Francesco, Francesco D'Andrea, Francesco Ferroni, Letizia Gardin, Chiara Salzillo, Rosa Nicoletti, Gianfranco Ferraro, Giuseppe A. Int J Biol Sci Research Paper Background: Lipomas are benign solid tumours that develop in soft tissues with origin in mesenchymal progenitors. Macroscopically, they appear as soft-elastic nodules, varying in volume from a few millimiters to several centimetres and can enlarge progressively. Although they are usually asymptomatic, they can cause symptoms due to nerve or vessel compression. Microscopically they appear as fibrous connective tissue stroma with embedded adipocytes, and absence of inflammation. Up to now no characterisation of stem cell population present in this tissue has been performed. Methods: Cytofluorimetric, biological and molecular biology analyses have been performed in order to test superficial cell markers and gene expression profile related to stemness and apoptotic activity of cells present in lipoma tissues compared to those of adipose tissue's cells. Results: Our results confirmed that CD34(+) cells in lipoma were present around small adipocytes, showing several altered biological activity such as proliferation, apoptotis and stemness. Conclusions: The data emerging from the comparison of the lipoma cells and normal adipose tissue, suggests the presence of cell precursors involved in the development of the lipoma. This hypothesis requires further investigation and may indicate new thresholds in the study of benign tumour pathogenesis. Ivyspring International Publisher 2015-07-18 /pmc/articles/PMC4551749/ /pubmed/26327807 http://dx.doi.org/10.7150/ijbs.11946 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions.
spellingShingle Research Paper
Zavan, Barbara
De Francesco, Francesco
D'Andrea, Francesco
Ferroni, Letizia
Gardin, Chiara
Salzillo, Rosa
Nicoletti, Gianfranco
Ferraro, Giuseppe A.
Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title_full Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title_fullStr Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title_full_unstemmed Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title_short Persistence of CD34 Stem Marker in Human Lipoma: Searching for Cancer Stem Cells
title_sort persistence of cd34 stem marker in human lipoma: searching for cancer stem cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551749/
https://www.ncbi.nlm.nih.gov/pubmed/26327807
http://dx.doi.org/10.7150/ijbs.11946
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