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Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle

The genome sequence of more than 100 Pseudomonas syringae strains has been sequenced to date; however only few of them have been fully assembled, including P. syringae pv. syringae B728a. Different strains of pv. syringae cause different diseases and have different host specificities; so, UMAF0158 i...

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Autores principales: Martínez-García, Pedro Manuel, Rodríguez-Palenzuela, Pablo, Arrebola, Eva, Carrión, Víctor J., Gutiérrez-Barranquero, José Antonio, Pérez-García, Alejandro, Ramos, Cayo, Cazorla, Francisco M., de Vicente, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551802/
https://www.ncbi.nlm.nih.gov/pubmed/26313942
http://dx.doi.org/10.1371/journal.pone.0136101
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author Martínez-García, Pedro Manuel
Rodríguez-Palenzuela, Pablo
Arrebola, Eva
Carrión, Víctor J.
Gutiérrez-Barranquero, José Antonio
Pérez-García, Alejandro
Ramos, Cayo
Cazorla, Francisco M.
de Vicente, Antonio
author_facet Martínez-García, Pedro Manuel
Rodríguez-Palenzuela, Pablo
Arrebola, Eva
Carrión, Víctor J.
Gutiérrez-Barranquero, José Antonio
Pérez-García, Alejandro
Ramos, Cayo
Cazorla, Francisco M.
de Vicente, Antonio
author_sort Martínez-García, Pedro Manuel
collection PubMed
description The genome sequence of more than 100 Pseudomonas syringae strains has been sequenced to date; however only few of them have been fully assembled, including P. syringae pv. syringae B728a. Different strains of pv. syringae cause different diseases and have different host specificities; so, UMAF0158 is a P. syringae pv. syringae strain related to B728a but instead of being a bean pathogen it causes apical necrosis of mango trees, and the two strains belong to different phylotypes of pv.syringae and clades of P. syringae. In this study we report the complete sequence and annotation of P. syringae pv. syringae UMAF0158 chromosome and plasmid pPSS158. A comparative analysis with the available sequenced genomes of other 25 P. syringae strains, both closed (the reference genomes DC3000, 1448A and B728a) and draft genomes was performed. The 5.8 Mb UMAF0158 chromosome has 59.3% GC content and comprises 5017 predicted protein-coding genes. Bioinformatics analysis revealed the presence of genes potentially implicated in the virulence and epiphytic fitness of this strain. We identified several genetic features, which are absent in B728a, that may explain the ability of UMAF0158 to colonize and infect mango trees: the mangotoxin biosynthetic operon mbo, a gene cluster for cellulose production, two different type III and two type VI secretion systems, and a particular T3SS effector repertoire. A mutant strain defective in the rhizobial-like T3SS Rhc showed no differences compared to wild-type during its interaction with host and non-host plants and worms. Here we report the first complete sequence of the chromosome of a pv. syringae strain pathogenic to a woody plant host. Our data also shed light on the genetic factors that possibly determine the pathogenic and epiphytic lifestyle of UMAF0158. This work provides the basis for further analysis on specific mechanisms that enable this strain to infect woody plants and for the functional analysis of host specificity in the P. syringae complex.
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spelling pubmed-45518022015-09-01 Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle Martínez-García, Pedro Manuel Rodríguez-Palenzuela, Pablo Arrebola, Eva Carrión, Víctor J. Gutiérrez-Barranquero, José Antonio Pérez-García, Alejandro Ramos, Cayo Cazorla, Francisco M. de Vicente, Antonio PLoS One Research Article The genome sequence of more than 100 Pseudomonas syringae strains has been sequenced to date; however only few of them have been fully assembled, including P. syringae pv. syringae B728a. Different strains of pv. syringae cause different diseases and have different host specificities; so, UMAF0158 is a P. syringae pv. syringae strain related to B728a but instead of being a bean pathogen it causes apical necrosis of mango trees, and the two strains belong to different phylotypes of pv.syringae and clades of P. syringae. In this study we report the complete sequence and annotation of P. syringae pv. syringae UMAF0158 chromosome and plasmid pPSS158. A comparative analysis with the available sequenced genomes of other 25 P. syringae strains, both closed (the reference genomes DC3000, 1448A and B728a) and draft genomes was performed. The 5.8 Mb UMAF0158 chromosome has 59.3% GC content and comprises 5017 predicted protein-coding genes. Bioinformatics analysis revealed the presence of genes potentially implicated in the virulence and epiphytic fitness of this strain. We identified several genetic features, which are absent in B728a, that may explain the ability of UMAF0158 to colonize and infect mango trees: the mangotoxin biosynthetic operon mbo, a gene cluster for cellulose production, two different type III and two type VI secretion systems, and a particular T3SS effector repertoire. A mutant strain defective in the rhizobial-like T3SS Rhc showed no differences compared to wild-type during its interaction with host and non-host plants and worms. Here we report the first complete sequence of the chromosome of a pv. syringae strain pathogenic to a woody plant host. Our data also shed light on the genetic factors that possibly determine the pathogenic and epiphytic lifestyle of UMAF0158. This work provides the basis for further analysis on specific mechanisms that enable this strain to infect woody plants and for the functional analysis of host specificity in the P. syringae complex. Public Library of Science 2015-08-27 /pmc/articles/PMC4551802/ /pubmed/26313942 http://dx.doi.org/10.1371/journal.pone.0136101 Text en © 2015 Martínez-García et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Martínez-García, Pedro Manuel
Rodríguez-Palenzuela, Pablo
Arrebola, Eva
Carrión, Víctor J.
Gutiérrez-Barranquero, José Antonio
Pérez-García, Alejandro
Ramos, Cayo
Cazorla, Francisco M.
de Vicente, Antonio
Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title_full Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title_fullStr Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title_full_unstemmed Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title_short Bioinformatics Analysis of the Complete Genome Sequence of the Mango Tree Pathogen Pseudomonas syringae pv. syringae UMAF0158 Reveals Traits Relevant to Virulence and Epiphytic Lifestyle
title_sort bioinformatics analysis of the complete genome sequence of the mango tree pathogen pseudomonas syringae pv. syringae umaf0158 reveals traits relevant to virulence and epiphytic lifestyle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4551802/
https://www.ncbi.nlm.nih.gov/pubmed/26313942
http://dx.doi.org/10.1371/journal.pone.0136101
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