Cargando…

Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds

BACKGROUND: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbit...

Descripción completa

Detalles Bibliográficos
Autores principales: Park, Jin-Young, Yang, Cheryl, Jung, Im-Hee, Lim, Hyun-Chang, Lee, Jung-Seok, Jung, Ui-Won, Seo, Young-Kwon, Park, Jung-Keug, Choi, Seong-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552159/
https://www.ncbi.nlm.nih.gov/pubmed/26331078
http://dx.doi.org/10.1186/s40824-015-0027-1
_version_ 1782387688808120320
author Park, Jin-Young
Yang, Cheryl
Jung, Im-Hee
Lim, Hyun-Chang
Lee, Jung-Seok
Jung, Ui-Won
Seo, Young-Kwon
Park, Jung-Keug
Choi, Seong-Ho
author_facet Park, Jin-Young
Yang, Cheryl
Jung, Im-Hee
Lim, Hyun-Chang
Lee, Jung-Seok
Jung, Ui-Won
Seo, Young-Kwon
Park, Jung-Keug
Choi, Seong-Ho
author_sort Park, Jin-Young
collection PubMed
description BACKGROUND: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis. RESULT: Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds mostly unresorbed at 4 weeks. The SEM observations after cell-seeding revealed attachment of the cells onto silk fibroin with production of extracellular matrix. New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone. There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups. CONCLUSION: Within the limitations of this study, silk scaffold is a biocompatible material with potential expediency as an osteoconductive scaffold in bone tissue engineering. However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis.
format Online
Article
Text
id pubmed-4552159
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45521592015-09-01 Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds Park, Jin-Young Yang, Cheryl Jung, Im-Hee Lim, Hyun-Chang Lee, Jung-Seok Jung, Ui-Won Seo, Young-Kwon Park, Jung-Keug Choi, Seong-Ho Biomater Res Research Article BACKGROUND: The aim of this study was to characterize the efficacy of nano-hydroxyapatite-coated silk fibroin constructs as a scaffold for bone tissue engineering and to determine the osteogenic effect of human dental pulp and periodontal ligament derived cells at an early stage of healing in rabbits. 3D silk fibroin constructs were developed and coated using nano-hydroxyapatite crystals. Dental pulp and periodontal ligament cells from extracted human third molars were cultured and seeded onto the silk scaffolds prior to in vivo implantation into 8 male New Zealand White rabbits. Four circular windows 8 mm in diameter were created in the calvarium of each animal. The defects were randomly allocated to the groups; (1) silk scaffold with dental pulp cells (DPSS), (2) silk scaffold with PDL cells (PDLSS), (3) normal saline-soaked silk scaffold (SS), and (4) empty control. The animals were sacrificed 2 (n = 4) or 4 weeks (n = 4) postoperatively. The characteristics of the silk scaffolds before and after cell seeding were analyzed using SEM. Samples were collected for histologic and histomorphometic analysis. ANOVA was used for statistical analysis. RESULT: Histologic view of the experimental sites showed well-maintained structure of the silk scaffolds mostly unresorbed at 4 weeks. The SEM observations after cell-seeding revealed attachment of the cells onto silk fibroin with production of extracellular matrix. New bone formation was observed in the 4 week groups occurring from the periphery of the defects and the silk fibers were closely integrated with the new bone. There was no significant difference in the amount of bone formation between the SS group and the DPSS and PDLSS groups. CONCLUSION: Within the limitations of this study, silk scaffold is a biocompatible material with potential expediency as an osteoconductive scaffold in bone tissue engineering. However, there was no evidence to suggest that the addition of hDPCs and hPDLCs to the current rabbit calvarial defect model can produce an early effect in augmenting osteogenesis. BioMed Central 2015-03-21 /pmc/articles/PMC4552159/ /pubmed/26331078 http://dx.doi.org/10.1186/s40824-015-0027-1 Text en © Park et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Park, Jin-Young
Yang, Cheryl
Jung, Im-Hee
Lim, Hyun-Chang
Lee, Jung-Seok
Jung, Ui-Won
Seo, Young-Kwon
Park, Jung-Keug
Choi, Seong-Ho
Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title_full Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title_fullStr Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title_full_unstemmed Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title_short Regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
title_sort regeneration of rabbit calvarial defects using cells-implanted nano-hydroxyapatite coated silk scaffolds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552159/
https://www.ncbi.nlm.nih.gov/pubmed/26331078
http://dx.doi.org/10.1186/s40824-015-0027-1
work_keys_str_mv AT parkjinyoung regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT yangcheryl regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT jungimhee regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT limhyunchang regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT leejungseok regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT junguiwon regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT seoyoungkwon regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT parkjungkeug regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds
AT choiseongho regenerationofrabbitcalvarialdefectsusingcellsimplantednanohydroxyapatitecoatedsilkscaffolds