Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe

BACKGROUND: Protein cage nanoparticles are promising nanoplatform candidates for efficient delivery systems of diagnostics and/or therapeutics because of their uniform size and structure as well as high biocompatibility and biodegradability. Encapsulin protein cage nanoparticle is used to develop a...

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Autores principales: Moon, Hyojin, Lee, Jisu, Kim, Hansol, Heo, Somin, Min, Junseon, Kang, Sebyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552281/
https://www.ncbi.nlm.nih.gov/pubmed/26331071
http://dx.doi.org/10.1186/2055-7124-18-21
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author Moon, Hyojin
Lee, Jisu
Kim, Hansol
Heo, Somin
Min, Junseon
Kang, Sebyung
author_facet Moon, Hyojin
Lee, Jisu
Kim, Hansol
Heo, Somin
Min, Junseon
Kang, Sebyung
author_sort Moon, Hyojin
collection PubMed
description BACKGROUND: Protein cage nanoparticles are promising nanoplatform candidates for efficient delivery systems of diagnostics and/or therapeutics because of their uniform size and structure as well as high biocompatibility and biodegradability. Encapsulin protein cage nanoparticle is used to develop a cell-specific targeting optical nanoprobe. RESULTS: FcBPs are genetically inserted and successfully displayed on the surface of encapsulin to form FcBP-encapsulin. Selectively binding of FcBP-encapsulin to SCC-7 is visualized with fluorescent microscopy. CONCLUSIONS: Encapsulin protein cage nanoparticle is robust enough to maintain their structure at high temperature and easily acquires multifunctions on demand through the combination of genetic and chemical modifications.
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spelling pubmed-45522812015-09-01 Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe Moon, Hyojin Lee, Jisu Kim, Hansol Heo, Somin Min, Junseon Kang, Sebyung Biomater Res Research Article BACKGROUND: Protein cage nanoparticles are promising nanoplatform candidates for efficient delivery systems of diagnostics and/or therapeutics because of their uniform size and structure as well as high biocompatibility and biodegradability. Encapsulin protein cage nanoparticle is used to develop a cell-specific targeting optical nanoprobe. RESULTS: FcBPs are genetically inserted and successfully displayed on the surface of encapsulin to form FcBP-encapsulin. Selectively binding of FcBP-encapsulin to SCC-7 is visualized with fluorescent microscopy. CONCLUSIONS: Encapsulin protein cage nanoparticle is robust enough to maintain their structure at high temperature and easily acquires multifunctions on demand through the combination of genetic and chemical modifications. BioMed Central 2014-12-23 /pmc/articles/PMC4552281/ /pubmed/26331071 http://dx.doi.org/10.1186/2055-7124-18-21 Text en © Moon et al.; licensee BioMed Central. 2014 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Moon, Hyojin
Lee, Jisu
Kim, Hansol
Heo, Somin
Min, Junseon
Kang, Sebyung
Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title_full Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title_fullStr Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title_full_unstemmed Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title_short Genetically engineering encapsulin protein cage nanoparticle as a SCC-7 cell targeting optical nanoprobe
title_sort genetically engineering encapsulin protein cage nanoparticle as a scc-7 cell targeting optical nanoprobe
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552281/
https://www.ncbi.nlm.nih.gov/pubmed/26331071
http://dx.doi.org/10.1186/2055-7124-18-21
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