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Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration

BACKGROUND: Angiogenin (ANG) is a potent stimulator of angiogenesis. The aim of this study was to fabricate an ANG-loaded scaffold and to evaluate its angiogenic and osteogenic effects. In this study, we fabricated an ANG-loaded scaffold using bovine bone powder and fibrin glue. We then evaluated th...

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Autores principales: Kim, Beom-Su, Kim, Jin-Seong, Yang, Sun-Sik, Kim, Hyung-Woo, Lim, Hun Jun, Lee, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552407/
https://www.ncbi.nlm.nih.gov/pubmed/26331087
http://dx.doi.org/10.1186/s40824-015-0040-4
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author Kim, Beom-Su
Kim, Jin-Seong
Yang, Sun-Sik
Kim, Hyung-Woo
Lim, Hun Jun
Lee, Jun
author_facet Kim, Beom-Su
Kim, Jin-Seong
Yang, Sun-Sik
Kim, Hyung-Woo
Lim, Hun Jun
Lee, Jun
author_sort Kim, Beom-Su
collection PubMed
description BACKGROUND: Angiogenin (ANG) is a potent stimulator of angiogenesis. The aim of this study was to fabricate an ANG-loaded scaffold and to evaluate its angiogenic and osteogenic effects. In this study, we fabricated an ANG-loaded scaffold using bovine bone powder and fibrin glue. We then evaluated the structural, morphological, and mechanical properties of the scaffold and the in vitro release profile of ANG. Cell proliferation, viability, and adhesion were evaluated using endothelial cells in vitro, and angiogenesis and new bone formation were evaluated using a rabbit calvarial defect model in vivo. RESULTS: Micro-computed tomography imaging showed that the bone powder was uniformly distributed in the scaffold, and scanning electron microscopy showed that the bone powder was bridged by polymerized fibrin. The porosity and compressive strength of the scaffolds were ~60 % and ~0.9 MPa, respectively, and were not significantly altered by ANG loading. In vitro, at 7 days, approximately 0.4 μg and 1.3 μg of the ANG were released from the FB/ANG 0.5 and FB/ANG 2.0, respectively and sustained slow release was observed until 25 days. The released ANG stimulated cell proliferation and adherence and was not cytotoxic. Furthermore, in vivo implantation resulted in enhanced angiogenesis, and new bone formation depended on the amount of loaded ANG. CONCLUSIONS: These studies demonstrate that a fibrin and bone powder scaffold loaded with ANG might be useful to promote bone regeneration by enhanced angiogenesis.
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spelling pubmed-45524072015-09-01 Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration Kim, Beom-Su Kim, Jin-Seong Yang, Sun-Sik Kim, Hyung-Woo Lim, Hun Jun Lee, Jun Biomater Res Research Article BACKGROUND: Angiogenin (ANG) is a potent stimulator of angiogenesis. The aim of this study was to fabricate an ANG-loaded scaffold and to evaluate its angiogenic and osteogenic effects. In this study, we fabricated an ANG-loaded scaffold using bovine bone powder and fibrin glue. We then evaluated the structural, morphological, and mechanical properties of the scaffold and the in vitro release profile of ANG. Cell proliferation, viability, and adhesion were evaluated using endothelial cells in vitro, and angiogenesis and new bone formation were evaluated using a rabbit calvarial defect model in vivo. RESULTS: Micro-computed tomography imaging showed that the bone powder was uniformly distributed in the scaffold, and scanning electron microscopy showed that the bone powder was bridged by polymerized fibrin. The porosity and compressive strength of the scaffolds were ~60 % and ~0.9 MPa, respectively, and were not significantly altered by ANG loading. In vitro, at 7 days, approximately 0.4 μg and 1.3 μg of the ANG were released from the FB/ANG 0.5 and FB/ANG 2.0, respectively and sustained slow release was observed until 25 days. The released ANG stimulated cell proliferation and adherence and was not cytotoxic. Furthermore, in vivo implantation resulted in enhanced angiogenesis, and new bone formation depended on the amount of loaded ANG. CONCLUSIONS: These studies demonstrate that a fibrin and bone powder scaffold loaded with ANG might be useful to promote bone regeneration by enhanced angiogenesis. BioMed Central 2015-08-25 /pmc/articles/PMC4552407/ /pubmed/26331087 http://dx.doi.org/10.1186/s40824-015-0040-4 Text en © Kim et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Kim, Beom-Su
Kim, Jin-Seong
Yang, Sun-Sik
Kim, Hyung-Woo
Lim, Hun Jun
Lee, Jun
Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title_full Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title_fullStr Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title_full_unstemmed Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title_short Angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
title_sort angiogenin-loaded fibrin/bone powder composite scaffold for vascularized bone regeneration
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552407/
https://www.ncbi.nlm.nih.gov/pubmed/26331087
http://dx.doi.org/10.1186/s40824-015-0040-4
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