Cargando…
Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects
BACKGROUND: In-vitro and animal studies using EDC cross-linked membranes have shown great resistance to enzymatic digestion as well as low cytotoxicity, and indicated its potential expediency as a barrier membrane for guided bone regeneration (GBR). The purpose of this study was to evaluate the effi...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552459/ https://www.ncbi.nlm.nih.gov/pubmed/26331084 http://dx.doi.org/10.1186/s40824-015-0038-y |
_version_ | 1782387731147522048 |
---|---|
author | Park, Jin-Young Jung, Im-Hee Kim, You-Kyoung Lim, Hyun-Chang Lee, Jung-Seok Jung, Ui-Won Choi, Seong-Ho |
author_facet | Park, Jin-Young Jung, Im-Hee Kim, You-Kyoung Lim, Hyun-Chang Lee, Jung-Seok Jung, Ui-Won Choi, Seong-Ho |
author_sort | Park, Jin-Young |
collection | PubMed |
description | BACKGROUND: In-vitro and animal studies using EDC cross-linked membranes have shown great resistance to enzymatic digestion as well as low cytotoxicity, and indicated its potential expediency as a barrier membrane for guided bone regeneration (GBR). The purpose of this study was to evaluate the efficacy, biocompatibility and degradation kinetics of a novel 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type I collagen membrane for regeneration of rabbit calvarial defects. EDC cross-linked type I collagen membrane and macroporous biphasic calcium phosphate (MBCP) consisting of 60 % hydroxyapatite and 40 % β-tricalcium phosphate were used in this study. Four circular defects (ø = 8 mm) were created in each calvarium of 12 male white rabbits. The experimental groups randomly allocated to the defects were as follows – (1) sham control, (2) EDC-cross-linked collagen membrane (EDC membrane), (3) bone graft (BG), and (4) bone graft with collagen membrane (B-EDC membrane). Specimens were harvested at 2 weeks (n = 6) and 8 weeks (n = 6) postoperatively for observational histology and histometrical analysis. RESULT: The histologic observation showed close adaptation of the EDC membrane to the defect perimeters along with vascularization of the membrane at 2 weeks. Direct apposition of new bone on to the collagen matrix could be observed displaying adequate tissue integration. Collapsing of the central portion of the membrane could be seen in the EDC membrane group, and both BG and B-EDC membrane groups showed greater total augmented area and new bone area than the EDC membrane group. The membrane was largely unresorbed at 2 weeks; and at 8 weeks the overall shape of the membrane was still maintained suggesting sustained barrier function at 8 weeks. CONCLUSION: Within the limits of this study, it may be concluded that EDC-cross-linked collagen membrane is a safe biomaterial with adequate tissue integration and resorption kinetics to support bone regeneration when used in conjunction with bone filler. |
format | Online Article Text |
id | pubmed-4552459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45524592015-09-01 Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects Park, Jin-Young Jung, Im-Hee Kim, You-Kyoung Lim, Hyun-Chang Lee, Jung-Seok Jung, Ui-Won Choi, Seong-Ho Biomater Res Research Article BACKGROUND: In-vitro and animal studies using EDC cross-linked membranes have shown great resistance to enzymatic digestion as well as low cytotoxicity, and indicated its potential expediency as a barrier membrane for guided bone regeneration (GBR). The purpose of this study was to evaluate the efficacy, biocompatibility and degradation kinetics of a novel 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type I collagen membrane for regeneration of rabbit calvarial defects. EDC cross-linked type I collagen membrane and macroporous biphasic calcium phosphate (MBCP) consisting of 60 % hydroxyapatite and 40 % β-tricalcium phosphate were used in this study. Four circular defects (ø = 8 mm) were created in each calvarium of 12 male white rabbits. The experimental groups randomly allocated to the defects were as follows – (1) sham control, (2) EDC-cross-linked collagen membrane (EDC membrane), (3) bone graft (BG), and (4) bone graft with collagen membrane (B-EDC membrane). Specimens were harvested at 2 weeks (n = 6) and 8 weeks (n = 6) postoperatively for observational histology and histometrical analysis. RESULT: The histologic observation showed close adaptation of the EDC membrane to the defect perimeters along with vascularization of the membrane at 2 weeks. Direct apposition of new bone on to the collagen matrix could be observed displaying adequate tissue integration. Collapsing of the central portion of the membrane could be seen in the EDC membrane group, and both BG and B-EDC membrane groups showed greater total augmented area and new bone area than the EDC membrane group. The membrane was largely unresorbed at 2 weeks; and at 8 weeks the overall shape of the membrane was still maintained suggesting sustained barrier function at 8 weeks. CONCLUSION: Within the limits of this study, it may be concluded that EDC-cross-linked collagen membrane is a safe biomaterial with adequate tissue integration and resorption kinetics to support bone regeneration when used in conjunction with bone filler. BioMed Central 2015-07-30 /pmc/articles/PMC4552459/ /pubmed/26331084 http://dx.doi.org/10.1186/s40824-015-0038-y Text en © Park et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Park, Jin-Young Jung, Im-Hee Kim, You-Kyoung Lim, Hyun-Chang Lee, Jung-Seok Jung, Ui-Won Choi, Seong-Ho Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title | Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title_full | Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title_fullStr | Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title_full_unstemmed | Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title_short | Guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)-cross-linked type-I collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
title_sort | guided bone regeneration using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc)-cross-linked type-i collagen membrane with biphasic calcium phosphate at rabbit calvarial defects |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552459/ https://www.ncbi.nlm.nih.gov/pubmed/26331084 http://dx.doi.org/10.1186/s40824-015-0038-y |
work_keys_str_mv | AT parkjinyoung guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT jungimhee guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT kimyoukyoung guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT limhyunchang guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT leejungseok guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT junguiwon guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects AT choiseongho guidedboneregenerationusing1ethyl33dimethylaminopropylcarbodiimideedccrosslinkedtypeicollagenmembranewithbiphasiccalciumphosphateatrabbitcalvarialdefects |