Cargando…

Genomic Comparison of Translocating and Non-Translocating Escherichia coli

Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to t...

Descripción completa

Detalles Bibliográficos
Autores principales: Bachmann, Nathan L., Katouli, Mohammad, Polkinghorne, Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552563/
https://www.ncbi.nlm.nih.gov/pubmed/26317913
http://dx.doi.org/10.1371/journal.pone.0137131
_version_ 1782387747502161920
author Bachmann, Nathan L.
Katouli, Mohammad
Polkinghorne, Adam
author_facet Bachmann, Nathan L.
Katouli, Mohammad
Polkinghorne, Adam
author_sort Bachmann, Nathan L.
collection PubMed
description Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46–4) and adhering but non-translocating E. coli (73–89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is likely to be associated with host-specific translocation. Phylogenetic analysis revealed that the four TEC strains were distributed amongst three distinct E. coli phylogroups, which was supported by the presence of phylogroup specific fimbriae gene clusters. The genomic comparison has identified potential genes that can be targeted with knock-out experiments to further characterise the mechanisms of E. coli translocation.
format Online
Article
Text
id pubmed-4552563
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45525632015-09-10 Genomic Comparison of Translocating and Non-Translocating Escherichia coli Bachmann, Nathan L. Katouli, Mohammad Polkinghorne, Adam PLoS One Research Article Translocation of E. coli across the gut epithelium can result in fatal sepsis in post-surgical patients. In vitro and in vivo experiments have identified the existence of a novel pathotype of translocating E. coli (TEC) that employs an unknown mechanism for translocating across epithelial cells to the mesenteric lymph nodes and the blood stream in both humans and animal models. In this study the genomes of four TEC strains isolated from the mesenteric lymph nodes of a fatal case of hospitalised patient (HMLN-1), blood of pigs after experimental shock (PC-1) and after non-lethal haemorrhage in rats (KIC-1 and KIC-2) were sequenced in order to identify the genes associated with their adhesion and/or translocation. To facilitate the comparison, the genomes of a non-adhering, non-translocating E. coli (46–4) and adhering but non-translocating E. coli (73–89) were also sequenced and compared. Whole genome comparison revealed that three (HMLN-1, PC-1 and KIC-2) of the four TEC strains carried a genomic island that encodes a Type 6 Secretion System that may contribute to adhesion of the bacteria to gut epithelial cells. The human TEC strain HMLN-1 also carried the invasion ibeA gene, which was absent in the animal TEC strains and is likely to be associated with host-specific translocation. Phylogenetic analysis revealed that the four TEC strains were distributed amongst three distinct E. coli phylogroups, which was supported by the presence of phylogroup specific fimbriae gene clusters. The genomic comparison has identified potential genes that can be targeted with knock-out experiments to further characterise the mechanisms of E. coli translocation. Public Library of Science 2015-08-28 /pmc/articles/PMC4552563/ /pubmed/26317913 http://dx.doi.org/10.1371/journal.pone.0137131 Text en © 2015 Bachmann et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bachmann, Nathan L.
Katouli, Mohammad
Polkinghorne, Adam
Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_full Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_fullStr Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_full_unstemmed Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_short Genomic Comparison of Translocating and Non-Translocating Escherichia coli
title_sort genomic comparison of translocating and non-translocating escherichia coli
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552563/
https://www.ncbi.nlm.nih.gov/pubmed/26317913
http://dx.doi.org/10.1371/journal.pone.0137131
work_keys_str_mv AT bachmannnathanl genomiccomparisonoftranslocatingandnontranslocatingescherichiacoli
AT katoulimohammad genomiccomparisonoftranslocatingandnontranslocatingescherichiacoli
AT polkinghorneadam genomiccomparisonoftranslocatingandnontranslocatingescherichiacoli