Energy Sensing versus 2-Oxoglutarate Dependent ATPase Switch in the Control of Synechococcus P(II) Interaction with Its Targets NAGK and PipX

P(II) proteins constitute a superfamily of highly conserved signaling devices, common in all domains of life. Through binding of the metabolites ATP, ADP and 2-oxoglutarate (2-OG), they undergo conformational changes which allow them to regulate a variety of target proteins including enzymes, transport proteins and transcription factors. But, in reverse, these target proteins also modulate the metabolite sensing properties of P(II), as has been recently shown. We used this effect to refine our P(II) based Förster resonance energy transfer (FRET) sensor and amplify its sensitivity towards ADP. With this enhanced sensor setup we addressed the question whether the P(II) protein from the model organism Synechococcus elongatus autonomously switches into the ADP conformation through ATPase activity as proposed in a recently published model. The present study disproves ATPase activity as a relevant mechanism for the transition of P(II) into the ADP state. In the absence of 2-OG, only the ATP/ADP ratio and concentration of ADP directs the competitive interaction of P(II) with two targets, one of which preferentially binds P(II) in the ATP-state, the other in the ADP-state.