Cargando…

GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats

BACKGROUND: Chemical stimulation of white adipose tissue (WAT) induces adipose afferent reflex (AAR), and thereby causes a general sympathetic activation. Paraventricular nucleus (PVN) is important in control of sympathetic outflow. This study was designed to investigate the role of γ-aminobutyric a...

Descripción completa

Detalles Bibliográficos
Autores principales: Ding, Lei, Gao, Run, Xiong, Xiao-Qing, Gao, Xing-Ya, Chen, Qi, Li, Yue-Hua, Kang, Yu-Ming, Zhu, Guo-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552845/
https://www.ncbi.nlm.nih.gov/pubmed/26317425
http://dx.doi.org/10.1371/journal.pone.0136983
_version_ 1782387796958248960
author Ding, Lei
Gao, Run
Xiong, Xiao-Qing
Gao, Xing-Ya
Chen, Qi
Li, Yue-Hua
Kang, Yu-Ming
Zhu, Guo-Qing
author_facet Ding, Lei
Gao, Run
Xiong, Xiao-Qing
Gao, Xing-Ya
Chen, Qi
Li, Yue-Hua
Kang, Yu-Ming
Zhu, Guo-Qing
author_sort Ding, Lei
collection PubMed
description BACKGROUND: Chemical stimulation of white adipose tissue (WAT) induces adipose afferent reflex (AAR), and thereby causes a general sympathetic activation. Paraventricular nucleus (PVN) is important in control of sympathetic outflow. This study was designed to investigate the role of γ-aminobutyric acid (GABA) in PVN in regulating the AAR. METHODOLOGY/PRINCIPAL FINDINGS: Experiments were carried out in anesthetized rats. Renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) were continuously recorded. AAR was evaluated by the RSNA and MAP responses to electrical stimulation of the right epididymal WAT (eWAT) afferent nerve. Electrical stimulation of eWAT afferent nerve increase RSNA. Bilateral microinjection of the GABA(A) receptor agonist isoguvacine or the GABA(B) receptor agonist baclofen attenuated the AAR. The effect of isoguvacine on the AAR was greater than that of baclofen. The GABA(A) receptor antagonist gabazine enhanced the AAR, while the GABA(B) receptor antagonist CGP-35348 had no significant effect on the AAR. Bilateral PVN microinjection of vigabatrin, a selective GABA-transaminase inhibitor, to increase endogenous GABA levels in the PVN abolished the AAR. The inhibitory effect of vigabatrin on the AAR was attenuated by the pretreatment with gabazine or CGP-35348. Pretreatment with combined gabazine and CGP-35348 abolished the effects of vigabatrin. CONCLUSIONS: Activation of GABA(A) or GABA(B) receptors in the PVN inhibits the AAR. Blockade of GABA(A) receptors in the PVN enhances the AAR. Endogenous GABA in the PVN plays an important role in regulating the AAR.
format Online
Article
Text
id pubmed-4552845
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-45528452015-09-10 GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats Ding, Lei Gao, Run Xiong, Xiao-Qing Gao, Xing-Ya Chen, Qi Li, Yue-Hua Kang, Yu-Ming Zhu, Guo-Qing PLoS One Research Article BACKGROUND: Chemical stimulation of white adipose tissue (WAT) induces adipose afferent reflex (AAR), and thereby causes a general sympathetic activation. Paraventricular nucleus (PVN) is important in control of sympathetic outflow. This study was designed to investigate the role of γ-aminobutyric acid (GABA) in PVN in regulating the AAR. METHODOLOGY/PRINCIPAL FINDINGS: Experiments were carried out in anesthetized rats. Renal sympathetic nerve activity (RSNA) and mean arterial pressure (MAP) were continuously recorded. AAR was evaluated by the RSNA and MAP responses to electrical stimulation of the right epididymal WAT (eWAT) afferent nerve. Electrical stimulation of eWAT afferent nerve increase RSNA. Bilateral microinjection of the GABA(A) receptor agonist isoguvacine or the GABA(B) receptor agonist baclofen attenuated the AAR. The effect of isoguvacine on the AAR was greater than that of baclofen. The GABA(A) receptor antagonist gabazine enhanced the AAR, while the GABA(B) receptor antagonist CGP-35348 had no significant effect on the AAR. Bilateral PVN microinjection of vigabatrin, a selective GABA-transaminase inhibitor, to increase endogenous GABA levels in the PVN abolished the AAR. The inhibitory effect of vigabatrin on the AAR was attenuated by the pretreatment with gabazine or CGP-35348. Pretreatment with combined gabazine and CGP-35348 abolished the effects of vigabatrin. CONCLUSIONS: Activation of GABA(A) or GABA(B) receptors in the PVN inhibits the AAR. Blockade of GABA(A) receptors in the PVN enhances the AAR. Endogenous GABA in the PVN plays an important role in regulating the AAR. Public Library of Science 2015-08-28 /pmc/articles/PMC4552845/ /pubmed/26317425 http://dx.doi.org/10.1371/journal.pone.0136983 Text en © 2015 Ding et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ding, Lei
Gao, Run
Xiong, Xiao-Qing
Gao, Xing-Ya
Chen, Qi
Li, Yue-Hua
Kang, Yu-Ming
Zhu, Guo-Qing
GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title_full GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title_fullStr GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title_full_unstemmed GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title_short GABA in Paraventricular Nucleus Regulates Adipose Afferent Reflex in Rats
title_sort gaba in paraventricular nucleus regulates adipose afferent reflex in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552845/
https://www.ncbi.nlm.nih.gov/pubmed/26317425
http://dx.doi.org/10.1371/journal.pone.0136983
work_keys_str_mv AT dinglei gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT gaorun gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT xiongxiaoqing gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT gaoxingya gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT chenqi gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT liyuehua gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT kangyuming gabainparaventricularnucleusregulatesadiposeafferentreflexinrats
AT zhuguoqing gabainparaventricularnucleusregulatesadiposeafferentreflexinrats