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A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis
BACKGROUND: VICKZ (IGF2BP1,2,3/ZBP1/Vg1RBP/IMP1,2,3) proteins bind RNA and help regulate many RNA-mediated processes. In the midbrain region of early chick embryos, VICKZ is expressed in the neural folds and along the basal surface of the neural epithelium, but, upon neural tube closure, is down-reg...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552865/ https://www.ncbi.nlm.nih.gov/pubmed/26317350 http://dx.doi.org/10.1371/journal.pone.0136408 |
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author | Carmel, Michal Shoshkes Kahane, Nitza Oberman, Froma Miloslavski, Rachel Sela-Donenfeld, Dalit Kalcheim, Chaya Yisraeli, Joel K. |
author_facet | Carmel, Michal Shoshkes Kahane, Nitza Oberman, Froma Miloslavski, Rachel Sela-Donenfeld, Dalit Kalcheim, Chaya Yisraeli, Joel K. |
author_sort | Carmel, Michal Shoshkes |
collection | PubMed |
description | BACKGROUND: VICKZ (IGF2BP1,2,3/ZBP1/Vg1RBP/IMP1,2,3) proteins bind RNA and help regulate many RNA-mediated processes. In the midbrain region of early chick embryos, VICKZ is expressed in the neural folds and along the basal surface of the neural epithelium, but, upon neural tube closure, is down-regulated in prospective cranial neural crest (CNC) cells, concomitant with their emigration and epithelial-to-mesenchymal transition (EMT). Electroporation of constructs that modulate cVICKZ expression demonstrates that this down-regulation is both necessary and sufficient for CNC EMT. These results suggest that VICKZ down-regulation in CNC cell-autonomously promotes EMT and migration. Reduction of VICKZ throughout the embryo, however, inhibits CNC migration non-cell-autonomously, as judged by transplantation experiments in Xenopus embryos. RESULTS AND CONCLUSIONS: Given the positive role reported for VICKZ proteins in promoting cell migration of chick embryo fibroblasts and many types of cancer cells, we have begun to look for specific mRNAs that could mediate context-specific differences. We report here that the laminin receptor, integrin alpha 6, is down-regulated in the dorsal neural tube when CNC cells emigrate, this process is mediated by cVICKZ, and integrin alpha 6 mRNA is found in VICKZ ribonucleoprotein complexes. Significantly, prolonged inhibition of cVICKZ in either the neural tube or the nascent dermomyotome sheet, which also dynamically expresses cVICKZ, induces disruption of these epithelia. These data point to a previously unreported role for VICKZ in maintaining epithelial integrity. |
format | Online Article Text |
id | pubmed-4552865 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45528652015-09-10 A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis Carmel, Michal Shoshkes Kahane, Nitza Oberman, Froma Miloslavski, Rachel Sela-Donenfeld, Dalit Kalcheim, Chaya Yisraeli, Joel K. PLoS One Research Article BACKGROUND: VICKZ (IGF2BP1,2,3/ZBP1/Vg1RBP/IMP1,2,3) proteins bind RNA and help regulate many RNA-mediated processes. In the midbrain region of early chick embryos, VICKZ is expressed in the neural folds and along the basal surface of the neural epithelium, but, upon neural tube closure, is down-regulated in prospective cranial neural crest (CNC) cells, concomitant with their emigration and epithelial-to-mesenchymal transition (EMT). Electroporation of constructs that modulate cVICKZ expression demonstrates that this down-regulation is both necessary and sufficient for CNC EMT. These results suggest that VICKZ down-regulation in CNC cell-autonomously promotes EMT and migration. Reduction of VICKZ throughout the embryo, however, inhibits CNC migration non-cell-autonomously, as judged by transplantation experiments in Xenopus embryos. RESULTS AND CONCLUSIONS: Given the positive role reported for VICKZ proteins in promoting cell migration of chick embryo fibroblasts and many types of cancer cells, we have begun to look for specific mRNAs that could mediate context-specific differences. We report here that the laminin receptor, integrin alpha 6, is down-regulated in the dorsal neural tube when CNC cells emigrate, this process is mediated by cVICKZ, and integrin alpha 6 mRNA is found in VICKZ ribonucleoprotein complexes. Significantly, prolonged inhibition of cVICKZ in either the neural tube or the nascent dermomyotome sheet, which also dynamically expresses cVICKZ, induces disruption of these epithelia. These data point to a previously unreported role for VICKZ in maintaining epithelial integrity. Public Library of Science 2015-08-28 /pmc/articles/PMC4552865/ /pubmed/26317350 http://dx.doi.org/10.1371/journal.pone.0136408 Text en © 2015 Carmel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Carmel, Michal Shoshkes Kahane, Nitza Oberman, Froma Miloslavski, Rachel Sela-Donenfeld, Dalit Kalcheim, Chaya Yisraeli, Joel K. A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title | A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title_full | A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title_fullStr | A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title_full_unstemmed | A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title_short | A Novel Role for VICKZ Proteins in Maintaining Epithelial Integrity during Embryogenesis |
title_sort | novel role for vickz proteins in maintaining epithelial integrity during embryogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552865/ https://www.ncbi.nlm.nih.gov/pubmed/26317350 http://dx.doi.org/10.1371/journal.pone.0136408 |
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