Cargando…
Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of se...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552890/ https://www.ncbi.nlm.nih.gov/pubmed/26317985 http://dx.doi.org/10.1371/journal.pone.0136241 |
_version_ | 1782387807464980480 |
---|---|
author | Liu, Yiying Rzeszutek, Elzbieta van der Voort, Menno Wu, Cheng-Hsuan Thoen, Even Skaar, Ida Bulone, Vincent Dorrestein, Pieter C. Raaijmakers, Jos M. de Bruijn, Irene |
author_facet | Liu, Yiying Rzeszutek, Elzbieta van der Voort, Menno Wu, Cheng-Hsuan Thoen, Even Skaar, Ida Bulone, Vincent Dorrestein, Pieter C. Raaijmakers, Jos M. de Bruijn, Irene |
author_sort | Liu, Yiying |
collection | PubMed |
description | Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of several chemical control measures, new sustainable methods are needed to mitigate Saprolegnia infections in aquaculture. Here, PhyloChip-based community analyses showed that the Pseudomonadales, particularly Pseudomonas species, represent one of the largest bacterial orders associated with salmon eggs from a commercial hatchery. Among the Pseudomonas species isolated from salmon eggs, significantly more biosurfactant producers were retrieved from healthy salmon eggs than from Saprolegnia-infected eggs. Subsequent in vivo activity bioassays showed that Pseudomonas isolate H6 significantly reduced salmon egg mortality caused by Saprolegnia diclina. Live colony mass spectrometry showed that strain H6 produces a viscosin-like lipopeptide surfactant. This biosurfactant inhibited growth of Saprolegnia in vitro, but no significant protection of salmon eggs against Saprolegniosis was observed. These results indicate that live inocula of aquatic Pseudomonas strains, instead of their bioactive compound, can provide new (micro)biological and sustainable means to mitigate oomycete diseases in aquaculture. |
format | Online Article Text |
id | pubmed-4552890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45528902015-09-10 Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia Liu, Yiying Rzeszutek, Elzbieta van der Voort, Menno Wu, Cheng-Hsuan Thoen, Even Skaar, Ida Bulone, Vincent Dorrestein, Pieter C. Raaijmakers, Jos M. de Bruijn, Irene PLoS One Research Article Emerging fungal and oomycete pathogens are increasingly threatening animals and plants globally. Amongst oomycetes, Saprolegnia species adversely affect wild and cultivated populations of amphibians and fish, leading to substantial reductions in biodiversity and food productivity. With the ban of several chemical control measures, new sustainable methods are needed to mitigate Saprolegnia infections in aquaculture. Here, PhyloChip-based community analyses showed that the Pseudomonadales, particularly Pseudomonas species, represent one of the largest bacterial orders associated with salmon eggs from a commercial hatchery. Among the Pseudomonas species isolated from salmon eggs, significantly more biosurfactant producers were retrieved from healthy salmon eggs than from Saprolegnia-infected eggs. Subsequent in vivo activity bioassays showed that Pseudomonas isolate H6 significantly reduced salmon egg mortality caused by Saprolegnia diclina. Live colony mass spectrometry showed that strain H6 produces a viscosin-like lipopeptide surfactant. This biosurfactant inhibited growth of Saprolegnia in vitro, but no significant protection of salmon eggs against Saprolegniosis was observed. These results indicate that live inocula of aquatic Pseudomonas strains, instead of their bioactive compound, can provide new (micro)biological and sustainable means to mitigate oomycete diseases in aquaculture. Public Library of Science 2015-08-28 /pmc/articles/PMC4552890/ /pubmed/26317985 http://dx.doi.org/10.1371/journal.pone.0136241 Text en © 2015 Liu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Liu, Yiying Rzeszutek, Elzbieta van der Voort, Menno Wu, Cheng-Hsuan Thoen, Even Skaar, Ida Bulone, Vincent Dorrestein, Pieter C. Raaijmakers, Jos M. de Bruijn, Irene Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia |
title | Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
|
title_full | Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
|
title_fullStr | Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
|
title_full_unstemmed | Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
|
title_short | Diversity of Aquatic Pseudomonas Species and Their Activity against the Fish Pathogenic Oomycete Saprolegnia
|
title_sort | diversity of aquatic pseudomonas species and their activity against the fish pathogenic oomycete saprolegnia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4552890/ https://www.ncbi.nlm.nih.gov/pubmed/26317985 http://dx.doi.org/10.1371/journal.pone.0136241 |
work_keys_str_mv | AT liuyiying diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT rzeszutekelzbieta diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT vandervoortmenno diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT wuchenghsuan diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT thoeneven diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT skaarida diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT bulonevincent diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT dorresteinpieterc diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT raaijmakersjosm diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia AT debruijnirene diversityofaquaticpseudomonasspeciesandtheiractivityagainstthefishpathogenicoomycetesaprolegnia |