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Metaproteomics of complex microbial communities in biogas plants

Production of biogas from agricultural biomass or organic wastes is an important source of renewable energy. Although thousands of biogas plants (BGPs) are operating in Germany, there is still a significant potential to improve yields, e.g. from fibrous substrates. In addition, process stability sho...

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Autores principales: Heyer, Robert, Kohrs, Fabian, Reichl, Udo, Benndorf, Dirk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554464/
https://www.ncbi.nlm.nih.gov/pubmed/25874383
http://dx.doi.org/10.1111/1751-7915.12276
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author Heyer, Robert
Kohrs, Fabian
Reichl, Udo
Benndorf, Dirk
author_facet Heyer, Robert
Kohrs, Fabian
Reichl, Udo
Benndorf, Dirk
author_sort Heyer, Robert
collection PubMed
description Production of biogas from agricultural biomass or organic wastes is an important source of renewable energy. Although thousands of biogas plants (BGPs) are operating in Germany, there is still a significant potential to improve yields, e.g. from fibrous substrates. In addition, process stability should be optimized. Besides evaluating technical measures, improving our understanding of microbial communities involved into the biogas process is considered as key issue to achieve both goals. Microscopic and genetic approaches to analyse community composition provide valuable experimental data, but fail to detect presence of enzymes and overall metabolic activity of microbial communities. Therefore, metaproteomics can significantly contribute to elucidate critical steps in the conversion of biomass to methane as it delivers combined functional and phylogenetic data. Although metaproteomics analyses are challenged by sample impurities, sample complexity and redundant protein identification, and are still limited by the availability of genome sequences, recent studies have shown promising results. In the following, the workflow and potential pitfalls for metaproteomics of samples from full-scale BGP are discussed. In addition, the value of metaproteomics to contribute to the further advancement of microbial ecology is evaluated. Finally, synergistic effects expected when metaproteomics is combined with advanced imaging techniques, metagenomics, metatranscriptomics and metabolomics are addressed.
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spelling pubmed-45544642015-09-04 Metaproteomics of complex microbial communities in biogas plants Heyer, Robert Kohrs, Fabian Reichl, Udo Benndorf, Dirk Microb Biotechnol Minireview Production of biogas from agricultural biomass or organic wastes is an important source of renewable energy. Although thousands of biogas plants (BGPs) are operating in Germany, there is still a significant potential to improve yields, e.g. from fibrous substrates. In addition, process stability should be optimized. Besides evaluating technical measures, improving our understanding of microbial communities involved into the biogas process is considered as key issue to achieve both goals. Microscopic and genetic approaches to analyse community composition provide valuable experimental data, but fail to detect presence of enzymes and overall metabolic activity of microbial communities. Therefore, metaproteomics can significantly contribute to elucidate critical steps in the conversion of biomass to methane as it delivers combined functional and phylogenetic data. Although metaproteomics analyses are challenged by sample impurities, sample complexity and redundant protein identification, and are still limited by the availability of genome sequences, recent studies have shown promising results. In the following, the workflow and potential pitfalls for metaproteomics of samples from full-scale BGP are discussed. In addition, the value of metaproteomics to contribute to the further advancement of microbial ecology is evaluated. Finally, synergistic effects expected when metaproteomics is combined with advanced imaging techniques, metagenomics, metatranscriptomics and metabolomics are addressed. John Wiley & Sons, Ltd 2015-09 2015-04-15 /pmc/articles/PMC4554464/ /pubmed/25874383 http://dx.doi.org/10.1111/1751-7915.12276 Text en Journal compilation © 2015 John Wiley & Sons Ltd and Society for Applied Microbiology http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Minireview
Heyer, Robert
Kohrs, Fabian
Reichl, Udo
Benndorf, Dirk
Metaproteomics of complex microbial communities in biogas plants
title Metaproteomics of complex microbial communities in biogas plants
title_full Metaproteomics of complex microbial communities in biogas plants
title_fullStr Metaproteomics of complex microbial communities in biogas plants
title_full_unstemmed Metaproteomics of complex microbial communities in biogas plants
title_short Metaproteomics of complex microbial communities in biogas plants
title_sort metaproteomics of complex microbial communities in biogas plants
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554464/
https://www.ncbi.nlm.nih.gov/pubmed/25874383
http://dx.doi.org/10.1111/1751-7915.12276
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