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Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L)
The objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH(4)) and volatile fatty acids (VFA) production. Th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST)
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554851/ https://www.ncbi.nlm.nih.gov/pubmed/26323400 http://dx.doi.org/10.5713/ajas.13.0834 |
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author | Paengkoum, P. Phonmun, T. Liang, J. B. Huang, X. D. Tan, H. Y. Jahromi, M. F. |
author_facet | Paengkoum, P. Phonmun, T. Liang, J. B. Huang, X. D. Tan, H. Y. Jahromi, M. F. |
author_sort | Paengkoum, P. |
collection | PubMed |
description | The objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH(4)) and volatile fatty acids (VFA) production. The average molecular weight (M(w)) of the purified CT was 2,081 Da with a protein binding affinity of 0.69 (the amount needed to bind half the maximum bovine serum albumin). In vitro gas production declined by 0.409, 0.121, and 0.311, respectively, while CH(4) production decreased by 0.211, 0.353, and 0.549, respectively, with addition of 10, 20, and 30 mg CT/500 mg dry matter (DM) compared to the control (p<0.05). The effects of CT from mangosteen-peel on in vitro DM degradability (IVDMD) and in vitro N degradability was negative and linear (p<0.01). Total VFA, concentrations of acetic, propionic, butyric and isovaleric acids decreased linearly with increasing amount of CT. The aforementioned results show that protein binding affinity of CT from mangosteen-peel is lower than those reported for Leucaena forages, however, the former has stronger negative effect on IVDMD. Therefore, the use of mangosteen-peel as protein source and CH(4) mitigating agent in ruminant feed requires further investigations. |
format | Online Article Text |
id | pubmed-4554851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) |
record_format | MEDLINE/PubMed |
spelling | pubmed-45548512015-10-01 Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) Paengkoum, P. Phonmun, T. Liang, J. B. Huang, X. D. Tan, H. Y. Jahromi, M. F. Asian-Australas J Anim Sci Article The objectives of this study were to determine the molecular weight of condensed tannins (CT) extracted from mangosteen (Garcinia mangostana L) peel, its protein binding affinity and effects on fermentation parameters including total gas, methane (CH(4)) and volatile fatty acids (VFA) production. The average molecular weight (M(w)) of the purified CT was 2,081 Da with a protein binding affinity of 0.69 (the amount needed to bind half the maximum bovine serum albumin). In vitro gas production declined by 0.409, 0.121, and 0.311, respectively, while CH(4) production decreased by 0.211, 0.353, and 0.549, respectively, with addition of 10, 20, and 30 mg CT/500 mg dry matter (DM) compared to the control (p<0.05). The effects of CT from mangosteen-peel on in vitro DM degradability (IVDMD) and in vitro N degradability was negative and linear (p<0.01). Total VFA, concentrations of acetic, propionic, butyric and isovaleric acids decreased linearly with increasing amount of CT. The aforementioned results show that protein binding affinity of CT from mangosteen-peel is lower than those reported for Leucaena forages, however, the former has stronger negative effect on IVDMD. Therefore, the use of mangosteen-peel as protein source and CH(4) mitigating agent in ruminant feed requires further investigations. Asian-Australasian Association of Animal Production Societies (AAAP) and Korean Society of Animal Science and Technology (KSAST) 2015-10 /pmc/articles/PMC4554851/ /pubmed/26323400 http://dx.doi.org/10.5713/ajas.13.0834 Text en Copyright © 2015 by Asian-Australasian Journal of Animal Sciences |
spellingShingle | Article Paengkoum, P. Phonmun, T. Liang, J. B. Huang, X. D. Tan, H. Y. Jahromi, M. F. Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title_full | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title_fullStr | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title_full_unstemmed | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title_short | Molecular Weight, Protein Binding Affinity and Methane Mitigation of Condensed Tannins from Mangosteen-peel (Garcinia mangostana L) |
title_sort | molecular weight, protein binding affinity and methane mitigation of condensed tannins from mangosteen-peel (garcinia mangostana l) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554851/ https://www.ncbi.nlm.nih.gov/pubmed/26323400 http://dx.doi.org/10.5713/ajas.13.0834 |
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