Cargando…

Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions

INTRODUCTION: Anal squamous cell carcinoma is preceded by persistent infection with high-risk human papillomavirus (HPV) and the cancer precursor, high-grade squamous intraepithelial lesion (HSIL). Detection of specific HPV genotypes and HPV-related biomarkers may be an option for primary anal scree...

Descripción completa

Detalles Bibliográficos
Autores principales: Cornall, Alyssa M, Roberts, Jennifer M, Molano, Monica, Machalek, Dorothy A, Phillips, Samuel, Hillman, Richard J, Grulich, Andrew E, Jin, Fengyi, Poynten, I Mary, Templeton, David J, Garland, Suzanne M, Tabrizi, Sepehr N
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554896/
https://www.ncbi.nlm.nih.gov/pubmed/26310402
http://dx.doi.org/10.1136/bmjopen-2015-008439
_version_ 1782388098219376640
author Cornall, Alyssa M
Roberts, Jennifer M
Molano, Monica
Machalek, Dorothy A
Phillips, Samuel
Hillman, Richard J
Grulich, Andrew E
Jin, Fengyi
Poynten, I Mary
Templeton, David J
Garland, Suzanne M
Tabrizi, Sepehr N
author_facet Cornall, Alyssa M
Roberts, Jennifer M
Molano, Monica
Machalek, Dorothy A
Phillips, Samuel
Hillman, Richard J
Grulich, Andrew E
Jin, Fengyi
Poynten, I Mary
Templeton, David J
Garland, Suzanne M
Tabrizi, Sepehr N
author_sort Cornall, Alyssa M
collection PubMed
description INTRODUCTION: Anal squamous cell carcinoma is preceded by persistent infection with high-risk human papillomavirus (HPV) and the cancer precursor, high-grade squamous intraepithelial lesion (HSIL). Detection of specific HPV genotypes and HPV-related biomarkers may be an option for primary anal screening. However, more data on the natural history of HPV-related anal lesions are required. The outcomes from this study will enhance our understanding of the clinical and biological behaviour of HPV-related anal lesions and inform the development of future HPV genotype and/or biomarker screening tests. METHODS AND ANALYSIS: HIV-negative and HIV-positive men who have sex with men, aged 35 years and over, recruited from community-based settings in Sydney, Australia, attend 6 clinic visits over 3 years. At the first 5 visits, participants undergo a digital anorectal examination, an anal swab for HPV genotyping and anal cytology, and high-resolution anoscopy with directed biopsy of any visible abnormalities that are suggestive of any abnormality suspicious of SIL. Tissue sections from participants diagnosed with histologically confirmed HSIL at the baseline clinic visit will undergo laser capture microdissection, HPV detection and genotyping, and quantitation of CpG methylation in baseline and follow-up biopsies. Histological and cytological findings in combination with HPV genotyping data will be used to identify persistent HSIL. HSIL will be stratified as non-persistent and persistent based on their status at 12 months. The performance of HPV genotype and methylation status in predicting disease persistence at 12 months will be assessed, along with associations with HIV status and other covariates such as age. ETHICS AND DISSEMINATION: The St Vincent's Hospital Ethics Committee granted ethics approval for the study. Written informed consent is obtained from all individuals before any study-specific procedures are performed. Findings from this study will be disseminated to participants and the community through study newsletters, and through peer-reviewed publications and international conferences.
format Online
Article
Text
id pubmed-4554896
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BMJ Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-45548962015-09-03 Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions Cornall, Alyssa M Roberts, Jennifer M Molano, Monica Machalek, Dorothy A Phillips, Samuel Hillman, Richard J Grulich, Andrew E Jin, Fengyi Poynten, I Mary Templeton, David J Garland, Suzanne M Tabrizi, Sepehr N BMJ Open Research Methods INTRODUCTION: Anal squamous cell carcinoma is preceded by persistent infection with high-risk human papillomavirus (HPV) and the cancer precursor, high-grade squamous intraepithelial lesion (HSIL). Detection of specific HPV genotypes and HPV-related biomarkers may be an option for primary anal screening. However, more data on the natural history of HPV-related anal lesions are required. The outcomes from this study will enhance our understanding of the clinical and biological behaviour of HPV-related anal lesions and inform the development of future HPV genotype and/or biomarker screening tests. METHODS AND ANALYSIS: HIV-negative and HIV-positive men who have sex with men, aged 35 years and over, recruited from community-based settings in Sydney, Australia, attend 6 clinic visits over 3 years. At the first 5 visits, participants undergo a digital anorectal examination, an anal swab for HPV genotyping and anal cytology, and high-resolution anoscopy with directed biopsy of any visible abnormalities that are suggestive of any abnormality suspicious of SIL. Tissue sections from participants diagnosed with histologically confirmed HSIL at the baseline clinic visit will undergo laser capture microdissection, HPV detection and genotyping, and quantitation of CpG methylation in baseline and follow-up biopsies. Histological and cytological findings in combination with HPV genotyping data will be used to identify persistent HSIL. HSIL will be stratified as non-persistent and persistent based on their status at 12 months. The performance of HPV genotype and methylation status in predicting disease persistence at 12 months will be assessed, along with associations with HIV status and other covariates such as age. ETHICS AND DISSEMINATION: The St Vincent's Hospital Ethics Committee granted ethics approval for the study. Written informed consent is obtained from all individuals before any study-specific procedures are performed. Findings from this study will be disseminated to participants and the community through study newsletters, and through peer-reviewed publications and international conferences. BMJ Publishing Group 2015-08-26 /pmc/articles/PMC4554896/ /pubmed/26310402 http://dx.doi.org/10.1136/bmjopen-2015-008439 Text en Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Methods
Cornall, Alyssa M
Roberts, Jennifer M
Molano, Monica
Machalek, Dorothy A
Phillips, Samuel
Hillman, Richard J
Grulich, Andrew E
Jin, Fengyi
Poynten, I Mary
Templeton, David J
Garland, Suzanne M
Tabrizi, Sepehr N
Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title_full Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title_fullStr Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title_full_unstemmed Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title_short Laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
title_sort laser capture microdissection as a tool to evaluate human papillomavirus genotyping and methylation as biomarkers of persistence and progression of anal lesions
topic Research Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554896/
https://www.ncbi.nlm.nih.gov/pubmed/26310402
http://dx.doi.org/10.1136/bmjopen-2015-008439
work_keys_str_mv AT cornallalyssam lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT robertsjenniferm lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT molanomonica lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT machalekdorothya lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT phillipssamuel lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT hillmanrichardj lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT grulichandrewe lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT jinfengyi lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT poyntenimary lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT templetondavidj lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT garlandsuzannem lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions
AT tabrizisepehrn lasercapturemicrodissectionasatooltoevaluatehumanpapillomavirusgenotypingandmethylationasbiomarkersofpersistenceandprogressionofanallesions