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Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice
Cigarette smoke-induced chronic obstructive pulmonary disease is a very debilitating disease, with a very high prevalence worldwide, which results in a expressive economic and social burden. Therefore, new therapeutic approaches to treat these patients are of unquestionable relevance. The use of mes...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554986/ https://www.ncbi.nlm.nih.gov/pubmed/26322981 http://dx.doi.org/10.1371/journal.pone.0136942 |
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author | Peron, Jean Pierre Schatzmann de Brito, Auriléia Aparecida Pelatti, Mayra Brandão, Wesley Nogueira Vitoretti, Luana Beatriz Greiffo, Flávia Regina da Silveira, Elaine Cristina Oliveira-Junior, Manuel Carneiro Maluf, Mariangela Evangelista, Lucila Halpern, Silvio Nisenbaum, Marcelo Gil Perin, Paulo Czeresnia, Carlos Eduardo Câmara, Niels Olsen Saraiva Aimbire, Flávio Vieira, Rodolfo de Paula Zatz, Mayana Ligeiro de Oliveira, Ana Paula |
author_facet | Peron, Jean Pierre Schatzmann de Brito, Auriléia Aparecida Pelatti, Mayra Brandão, Wesley Nogueira Vitoretti, Luana Beatriz Greiffo, Flávia Regina da Silveira, Elaine Cristina Oliveira-Junior, Manuel Carneiro Maluf, Mariangela Evangelista, Lucila Halpern, Silvio Nisenbaum, Marcelo Gil Perin, Paulo Czeresnia, Carlos Eduardo Câmara, Niels Olsen Saraiva Aimbire, Flávio Vieira, Rodolfo de Paula Zatz, Mayana Ligeiro de Oliveira, Ana Paula |
author_sort | Peron, Jean Pierre Schatzmann |
collection | PubMed |
description | Cigarette smoke-induced chronic obstructive pulmonary disease is a very debilitating disease, with a very high prevalence worldwide, which results in a expressive economic and social burden. Therefore, new therapeutic approaches to treat these patients are of unquestionable relevance. The use of mesenchymal stromal cells (MSCs) is an innovative and yet accessible approach for pulmonary acute and chronic diseases, mainly due to its important immunoregulatory, anti-fibrogenic, anti-apoptotic and pro-angiogenic. Besides, the use of adjuvant therapies, whose aim is to boost or synergize with their function should be tested. Low level laser (LLL) therapy is a relatively new and promising approach, with very low cost, no invasiveness and no side effects. Here, we aimed to study the effectiveness of human tube derived MSCs (htMSCs) cell therapy associated with a 30mW/3J—660 nm LLL irradiation in experimental cigarette smoke-induced chronic obstructive pulmonary disease. Thus, C57BL/6 mice were exposed to cigarette smoke for 75 days (twice a day) and all experiments were performed on day 76. Experimental groups receive htMSCS either intraperitoneally or intranasally and/or LLL irradiation either alone or in association. We show that co-therapy greatly reduces lung inflammation, lowering the cellular infiltrate and pro-inflammatory cytokine secretion (IL-1β, IL-6, TNF-α and KC), which were followed by decreased mucus production, collagen accumulation and tissue damage. These findings seemed to be secondary to the reduction of both NF-κB and NF-AT activation in lung tissues with a concomitant increase in IL-10. In summary, our data suggests that the concomitant use of MSCs + LLLT may be a promising therapeutic approach for lung inflammatory diseases as COPD. |
format | Online Article Text |
id | pubmed-4554986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45549862015-09-10 Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice Peron, Jean Pierre Schatzmann de Brito, Auriléia Aparecida Pelatti, Mayra Brandão, Wesley Nogueira Vitoretti, Luana Beatriz Greiffo, Flávia Regina da Silveira, Elaine Cristina Oliveira-Junior, Manuel Carneiro Maluf, Mariangela Evangelista, Lucila Halpern, Silvio Nisenbaum, Marcelo Gil Perin, Paulo Czeresnia, Carlos Eduardo Câmara, Niels Olsen Saraiva Aimbire, Flávio Vieira, Rodolfo de Paula Zatz, Mayana Ligeiro de Oliveira, Ana Paula PLoS One Research Article Cigarette smoke-induced chronic obstructive pulmonary disease is a very debilitating disease, with a very high prevalence worldwide, which results in a expressive economic and social burden. Therefore, new therapeutic approaches to treat these patients are of unquestionable relevance. The use of mesenchymal stromal cells (MSCs) is an innovative and yet accessible approach for pulmonary acute and chronic diseases, mainly due to its important immunoregulatory, anti-fibrogenic, anti-apoptotic and pro-angiogenic. Besides, the use of adjuvant therapies, whose aim is to boost or synergize with their function should be tested. Low level laser (LLL) therapy is a relatively new and promising approach, with very low cost, no invasiveness and no side effects. Here, we aimed to study the effectiveness of human tube derived MSCs (htMSCs) cell therapy associated with a 30mW/3J—660 nm LLL irradiation in experimental cigarette smoke-induced chronic obstructive pulmonary disease. Thus, C57BL/6 mice were exposed to cigarette smoke for 75 days (twice a day) and all experiments were performed on day 76. Experimental groups receive htMSCS either intraperitoneally or intranasally and/or LLL irradiation either alone or in association. We show that co-therapy greatly reduces lung inflammation, lowering the cellular infiltrate and pro-inflammatory cytokine secretion (IL-1β, IL-6, TNF-α and KC), which were followed by decreased mucus production, collagen accumulation and tissue damage. These findings seemed to be secondary to the reduction of both NF-κB and NF-AT activation in lung tissues with a concomitant increase in IL-10. In summary, our data suggests that the concomitant use of MSCs + LLLT may be a promising therapeutic approach for lung inflammatory diseases as COPD. Public Library of Science 2015-08-31 /pmc/articles/PMC4554986/ /pubmed/26322981 http://dx.doi.org/10.1371/journal.pone.0136942 Text en © 2015 Peron et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Peron, Jean Pierre Schatzmann de Brito, Auriléia Aparecida Pelatti, Mayra Brandão, Wesley Nogueira Vitoretti, Luana Beatriz Greiffo, Flávia Regina da Silveira, Elaine Cristina Oliveira-Junior, Manuel Carneiro Maluf, Mariangela Evangelista, Lucila Halpern, Silvio Nisenbaum, Marcelo Gil Perin, Paulo Czeresnia, Carlos Eduardo Câmara, Niels Olsen Saraiva Aimbire, Flávio Vieira, Rodolfo de Paula Zatz, Mayana Ligeiro de Oliveira, Ana Paula Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title | Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title_full | Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title_fullStr | Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title_full_unstemmed | Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title_short | Human Tubal-Derived Mesenchymal Stromal Cells Associated with Low Level Laser Therapy Significantly Reduces Cigarette Smoke–Induced COPD in C57BL/6 mice |
title_sort | human tubal-derived mesenchymal stromal cells associated with low level laser therapy significantly reduces cigarette smoke–induced copd in c57bl/6 mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4554986/ https://www.ncbi.nlm.nih.gov/pubmed/26322981 http://dx.doi.org/10.1371/journal.pone.0136942 |
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