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Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)

The spatial interactions of synaptic vesicles in synapses were assessed after a detailed characterization of size, shape, and orientation of the synaptic vesicles. We hypothesized that shape and orientation of the synaptic vesicles are influenced by their movement toward the active zone causing devi...

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Autores principales: Khanmohammadi, Mahdieh, Waagepetersen, Rasmus P., Sporring, Jon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555025/
https://www.ncbi.nlm.nih.gov/pubmed/26388743
http://dx.doi.org/10.3389/fnana.2015.00116
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author Khanmohammadi, Mahdieh
Waagepetersen, Rasmus P.
Sporring, Jon
author_facet Khanmohammadi, Mahdieh
Waagepetersen, Rasmus P.
Sporring, Jon
author_sort Khanmohammadi, Mahdieh
collection PubMed
description The spatial interactions of synaptic vesicles in synapses were assessed after a detailed characterization of size, shape, and orientation of the synaptic vesicles. We hypothesized that shape and orientation of the synaptic vesicles are influenced by their movement toward the active zone causing deviations from spherical shape and systematic trends in their orientation. We studied three-dimensional representations of synapses obtained by manual annotation of focused ion beam scanning electron microscopy (FIB-SEM) images of male mouse brain. The configurations of synaptic vesicles were regarded as marked point patterns, where the points are the centers of the vesicles, and the mark of a vesicle is given by its size, shape, and orientation characteristics. Statistics for marked point processes were employed to study spatial interactions between vesicles. We found that the synaptic vesicles in excitatory synapses appeared to be of oblate ellipsoid shape and in inhibitory synapses appeared to be of cigar ellipsoid shape, and followed a systematic pattern regarding their orientation toward the active zone. Moreover, there was strong evidence of spatial alignment in the orientations of pairs of synaptic vesicles, and of repulsion between them only in excitatory synapses, beyond that caused by their physical extent.
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spelling pubmed-45550252015-09-18 Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM) Khanmohammadi, Mahdieh Waagepetersen, Rasmus P. Sporring, Jon Front Neuroanat Neuroscience The spatial interactions of synaptic vesicles in synapses were assessed after a detailed characterization of size, shape, and orientation of the synaptic vesicles. We hypothesized that shape and orientation of the synaptic vesicles are influenced by their movement toward the active zone causing deviations from spherical shape and systematic trends in their orientation. We studied three-dimensional representations of synapses obtained by manual annotation of focused ion beam scanning electron microscopy (FIB-SEM) images of male mouse brain. The configurations of synaptic vesicles were regarded as marked point patterns, where the points are the centers of the vesicles, and the mark of a vesicle is given by its size, shape, and orientation characteristics. Statistics for marked point processes were employed to study spatial interactions between vesicles. We found that the synaptic vesicles in excitatory synapses appeared to be of oblate ellipsoid shape and in inhibitory synapses appeared to be of cigar ellipsoid shape, and followed a systematic pattern regarding their orientation toward the active zone. Moreover, there was strong evidence of spatial alignment in the orientations of pairs of synaptic vesicles, and of repulsion between them only in excitatory synapses, beyond that caused by their physical extent. Frontiers Media S.A. 2015-09-01 /pmc/articles/PMC4555025/ /pubmed/26388743 http://dx.doi.org/10.3389/fnana.2015.00116 Text en Copyright © 2015 Khanmohammadi, Waagepetersen and Sporring. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Khanmohammadi, Mahdieh
Waagepetersen, Rasmus P.
Sporring, Jon
Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title_full Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title_fullStr Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title_full_unstemmed Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title_short Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
title_sort analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (fib-sem)
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555025/
https://www.ncbi.nlm.nih.gov/pubmed/26388743
http://dx.doi.org/10.3389/fnana.2015.00116
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