Cargando…

RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album

Santalum album (sandalwood) is one of the economically important plant species in the Santalaceae for its production of highly valued perfume oils. Sandalwood is also a hemiparasitic tree that obtains some of its water and simple nutrients by tapping into other plants through haustoria which are hig...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xinhua, Berkowitz, Oliver, Teixeira da Silva, Jaime A., Zhang, Muhan, Ma, Guohua, Whelan, James, Duan, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555033/
https://www.ncbi.nlm.nih.gov/pubmed/26388878
http://dx.doi.org/10.3389/fpls.2015.00661
_version_ 1782388119113302016
author Zhang, Xinhua
Berkowitz, Oliver
Teixeira da Silva, Jaime A.
Zhang, Muhan
Ma, Guohua
Whelan, James
Duan, Jun
author_facet Zhang, Xinhua
Berkowitz, Oliver
Teixeira da Silva, Jaime A.
Zhang, Muhan
Ma, Guohua
Whelan, James
Duan, Jun
author_sort Zhang, Xinhua
collection PubMed
description Santalum album (sandalwood) is one of the economically important plant species in the Santalaceae for its production of highly valued perfume oils. Sandalwood is also a hemiparasitic tree that obtains some of its water and simple nutrients by tapping into other plants through haustoria which are highly specialized organs in parasitic angiosperms. However, an understanding of the molecular mechanisms involved in haustorium development is limited. In this study, RNA sequencing (RNA-seq) analyses were performed to identify changes in gene expression and metabolic pathways associated with the development of the S. album haustorium. A total of 56,011 non-redundant contigs with a mean contig size of 618 bp were obtained by de novo assembly of the transcriptome of haustoria and non-haustorial seedling roots. A substantial number of the identified differentially expressed genes were involved in cell wall metabolism and protein metabolism, as well as mitochondrial electron transport functions. Phytohormone-mediated regulation might play an important role during haustorial development. Especially, auxin signaling is likely to be essential for haustorial initiation, and genes related to cytokinin and gibberellin biosynthesis and metabolism are involved in haustorial development. Our results suggest that genes encoding nodulin-like proteins may be important for haustorial morphogenesis in S. album. The obtained sequence data will become a rich resource for future research in this interesting species. This information improves our understanding of haustorium development in root hemiparasitic species and will allow further exploration of the detailed molecular mechanisms underlying plant parasitism.
format Online
Article
Text
id pubmed-4555033
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-45550332015-09-18 RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album Zhang, Xinhua Berkowitz, Oliver Teixeira da Silva, Jaime A. Zhang, Muhan Ma, Guohua Whelan, James Duan, Jun Front Plant Sci Plant Science Santalum album (sandalwood) is one of the economically important plant species in the Santalaceae for its production of highly valued perfume oils. Sandalwood is also a hemiparasitic tree that obtains some of its water and simple nutrients by tapping into other plants through haustoria which are highly specialized organs in parasitic angiosperms. However, an understanding of the molecular mechanisms involved in haustorium development is limited. In this study, RNA sequencing (RNA-seq) analyses were performed to identify changes in gene expression and metabolic pathways associated with the development of the S. album haustorium. A total of 56,011 non-redundant contigs with a mean contig size of 618 bp were obtained by de novo assembly of the transcriptome of haustoria and non-haustorial seedling roots. A substantial number of the identified differentially expressed genes were involved in cell wall metabolism and protein metabolism, as well as mitochondrial electron transport functions. Phytohormone-mediated regulation might play an important role during haustorial development. Especially, auxin signaling is likely to be essential for haustorial initiation, and genes related to cytokinin and gibberellin biosynthesis and metabolism are involved in haustorial development. Our results suggest that genes encoding nodulin-like proteins may be important for haustorial morphogenesis in S. album. The obtained sequence data will become a rich resource for future research in this interesting species. This information improves our understanding of haustorium development in root hemiparasitic species and will allow further exploration of the detailed molecular mechanisms underlying plant parasitism. Frontiers Media S.A. 2015-09-01 /pmc/articles/PMC4555033/ /pubmed/26388878 http://dx.doi.org/10.3389/fpls.2015.00661 Text en Copyright © 2015 Zhang, Berkowitz, Teixeira da Silva, Zhang, Ma, Whelan and Duan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Xinhua
Berkowitz, Oliver
Teixeira da Silva, Jaime A.
Zhang, Muhan
Ma, Guohua
Whelan, James
Duan, Jun
RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title_full RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title_fullStr RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title_full_unstemmed RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title_short RNA-Seq analysis identifies key genes associated with haustorial development in the root hemiparasite Santalum album
title_sort rna-seq analysis identifies key genes associated with haustorial development in the root hemiparasite santalum album
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555033/
https://www.ncbi.nlm.nih.gov/pubmed/26388878
http://dx.doi.org/10.3389/fpls.2015.00661
work_keys_str_mv AT zhangxinhua rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT berkowitzoliver rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT teixeiradasilvajaimea rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT zhangmuhan rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT maguohua rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT whelanjames rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum
AT duanjun rnaseqanalysisidentifieskeygenesassociatedwithhaustorialdevelopmentintheroothemiparasitesantalumalbum