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Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a recently emerged virus that has caused a number of human infections and deaths, primarily in the Middle East. The transmission of MERS-CoV to humans has been proposed to be as a result of contact with camels, but evidence of human-to-human...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V. Published by Elsevier B.V.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555185/ https://www.ncbi.nlm.nih.gov/pubmed/26190637 http://dx.doi.org/10.1016/j.jviromet.2015.07.002 |
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author | Kumar, Mia Mazur, Steven Ork, Britini L. Postnikova, Elena Hensley, Lisa E. Jahrling, Peter B. Johnson, Reed Holbrook, Michael R. |
author_facet | Kumar, Mia Mazur, Steven Ork, Britini L. Postnikova, Elena Hensley, Lisa E. Jahrling, Peter B. Johnson, Reed Holbrook, Michael R. |
author_sort | Kumar, Mia |
collection | PubMed |
description | Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a recently emerged virus that has caused a number of human infections and deaths, primarily in the Middle East. The transmission of MERS-CoV to humans has been proposed to be as a result of contact with camels, but evidence of human-to-human transmission also exists. In order to work with MERS-CoV in a laboratory setting, the US Centers for Disease Control and Prevention (CDC) has determined that MERS-CoV should be handled at a biosafety level (BSL) 3 (BSL-3) biocontainment level. Many processes and procedures used to characterize MERS-CoV and to evaluate samples from MERS-CoV infected animals are more easily and efficiently completed at BSL-2 or lower containment. In order to complete experimental work at BSL-2, demonstration or proof of inactivation is required before removal of specimens from biocontainment laboratories. In the studies presented here, we evaluated typical means of inactivating viruses prior to handling specimens at a lower biocontainment level. We found that Trizol, AVL buffer and gamma irradiation were effective at inactivating MERS-CoV, that formaldehyde-based solutions required at least 30 min of contact time in a cell culture system while a mixture of methanol and acetone required 60 min to inactivate MERS-CoV. Together, these data provide a foundation for safely inactivating MERS-CoV, and potentially other coronaviruses, prior to removal from biocontainment facilities. |
format | Online Article Text |
id | pubmed-4555185 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Elsevier B.V. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-45551852016-10-01 Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus Kumar, Mia Mazur, Steven Ork, Britini L. Postnikova, Elena Hensley, Lisa E. Jahrling, Peter B. Johnson, Reed Holbrook, Michael R. J Virol Methods Article Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a recently emerged virus that has caused a number of human infections and deaths, primarily in the Middle East. The transmission of MERS-CoV to humans has been proposed to be as a result of contact with camels, but evidence of human-to-human transmission also exists. In order to work with MERS-CoV in a laboratory setting, the US Centers for Disease Control and Prevention (CDC) has determined that MERS-CoV should be handled at a biosafety level (BSL) 3 (BSL-3) biocontainment level. Many processes and procedures used to characterize MERS-CoV and to evaluate samples from MERS-CoV infected animals are more easily and efficiently completed at BSL-2 or lower containment. In order to complete experimental work at BSL-2, demonstration or proof of inactivation is required before removal of specimens from biocontainment laboratories. In the studies presented here, we evaluated typical means of inactivating viruses prior to handling specimens at a lower biocontainment level. We found that Trizol, AVL buffer and gamma irradiation were effective at inactivating MERS-CoV, that formaldehyde-based solutions required at least 30 min of contact time in a cell culture system while a mixture of methanol and acetone required 60 min to inactivate MERS-CoV. Together, these data provide a foundation for safely inactivating MERS-CoV, and potentially other coronaviruses, prior to removal from biocontainment facilities. Elsevier B.V. Published by Elsevier B.V. 2015-10 2015-07-17 /pmc/articles/PMC4555185/ /pubmed/26190637 http://dx.doi.org/10.1016/j.jviromet.2015.07.002 Text en Copyright © 2015 Elsevier B.V. Published by Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Kumar, Mia Mazur, Steven Ork, Britini L. Postnikova, Elena Hensley, Lisa E. Jahrling, Peter B. Johnson, Reed Holbrook, Michael R. Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title | Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title_full | Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title_fullStr | Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title_full_unstemmed | Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title_short | Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus |
title_sort | inactivation and safety testing of middle east respiratory syndrome coronavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555185/ https://www.ncbi.nlm.nih.gov/pubmed/26190637 http://dx.doi.org/10.1016/j.jviromet.2015.07.002 |
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