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Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?

The growing global production of zinc oxide nanoparticles (ZnONPs) suggests a realistic increase in the environmental exposure to such a nanomaterial, making the knowledge of its biological reactivity and its safe-by-design synthesis mandatory. In this study, the embryotoxicity of ZnONPs (1–100 mg/L...

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Autores principales: Bonfanti, Patrizia, Moschini, Elisa, Saibene, Melissa, Bacchetta, Renato, Rettighieri, Leonardo, Calabri, Lorenzo, Colombo, Anita, Mantecca, Paride
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555250/
https://www.ncbi.nlm.nih.gov/pubmed/26225989
http://dx.doi.org/10.3390/ijerph120808828
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author Bonfanti, Patrizia
Moschini, Elisa
Saibene, Melissa
Bacchetta, Renato
Rettighieri, Leonardo
Calabri, Lorenzo
Colombo, Anita
Mantecca, Paride
author_facet Bonfanti, Patrizia
Moschini, Elisa
Saibene, Melissa
Bacchetta, Renato
Rettighieri, Leonardo
Calabri, Lorenzo
Colombo, Anita
Mantecca, Paride
author_sort Bonfanti, Patrizia
collection PubMed
description The growing global production of zinc oxide nanoparticles (ZnONPs) suggests a realistic increase in the environmental exposure to such a nanomaterial, making the knowledge of its biological reactivity and its safe-by-design synthesis mandatory. In this study, the embryotoxicity of ZnONPs (1–100 mg/L) specifically synthesized for industrial purposes with different sizes, shapes (round, rod) and surface coatings (PEG, PVP) was tested using the frog embryo teratogenesis assay-Xenopus (FETAX) to identify potential target tissues and the most sensitive developmental stages. The ZnONPs did not cause embryolethality, but induced a high incidence of malformations, in particular misfolded gut and abdominal edema. Smaller, round NPs were more effective than the bigger, rod ones, and PEGylation determined a reduction in embryotoxicity. Ingestion appeared to be the most relevant exposure route. Only the embryos exposed from the stomodeum opening showed anatomical and histological lesions to the intestine, mainly referable to a swelling of paracellular spaces among enterocytes. In conclusion, ZnONPs differing in shape and surface coating displayed similar toxicity in X. laevis embryos and shared the same target organ. Nevertheless, we cannot exclude that the physico-chemical characteristics may influence the severity of such effects. Further research efforts are mandatory to ensure the synthesis of safer nano-ZnO-containing products.
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spelling pubmed-45552502015-09-01 Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis? Bonfanti, Patrizia Moschini, Elisa Saibene, Melissa Bacchetta, Renato Rettighieri, Leonardo Calabri, Lorenzo Colombo, Anita Mantecca, Paride Int J Environ Res Public Health Article The growing global production of zinc oxide nanoparticles (ZnONPs) suggests a realistic increase in the environmental exposure to such a nanomaterial, making the knowledge of its biological reactivity and its safe-by-design synthesis mandatory. In this study, the embryotoxicity of ZnONPs (1–100 mg/L) specifically synthesized for industrial purposes with different sizes, shapes (round, rod) and surface coatings (PEG, PVP) was tested using the frog embryo teratogenesis assay-Xenopus (FETAX) to identify potential target tissues and the most sensitive developmental stages. The ZnONPs did not cause embryolethality, but induced a high incidence of malformations, in particular misfolded gut and abdominal edema. Smaller, round NPs were more effective than the bigger, rod ones, and PEGylation determined a reduction in embryotoxicity. Ingestion appeared to be the most relevant exposure route. Only the embryos exposed from the stomodeum opening showed anatomical and histological lesions to the intestine, mainly referable to a swelling of paracellular spaces among enterocytes. In conclusion, ZnONPs differing in shape and surface coating displayed similar toxicity in X. laevis embryos and shared the same target organ. Nevertheless, we cannot exclude that the physico-chemical characteristics may influence the severity of such effects. Further research efforts are mandatory to ensure the synthesis of safer nano-ZnO-containing products. MDPI 2015-07-28 2015-08 /pmc/articles/PMC4555250/ /pubmed/26225989 http://dx.doi.org/10.3390/ijerph120808828 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bonfanti, Patrizia
Moschini, Elisa
Saibene, Melissa
Bacchetta, Renato
Rettighieri, Leonardo
Calabri, Lorenzo
Colombo, Anita
Mantecca, Paride
Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title_full Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title_fullStr Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title_full_unstemmed Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title_short Do Nanoparticle Physico-Chemical Properties and Developmental Exposure Window Influence Nano ZnO Embryotoxicity in Xenopus laevis?
title_sort do nanoparticle physico-chemical properties and developmental exposure window influence nano zno embryotoxicity in xenopus laevis?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4555250/
https://www.ncbi.nlm.nih.gov/pubmed/26225989
http://dx.doi.org/10.3390/ijerph120808828
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