Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma

BACKGROUND: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated. METHODS: The Apoptosis was evidenced by investigating DNA fr...

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Autores principales: Boubaker, Jihed, Mokdad Bzeouich, Imen, Nasr, Nouha, Ben Ghozlen, Hajer, Mustapha, Nadia, Ghedira, Kamel, Chekir-Ghedira, Leila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556050/
https://www.ncbi.nlm.nih.gov/pubmed/26329604
http://dx.doi.org/10.1186/s12906-015-0743-z
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author Boubaker, Jihed
Mokdad Bzeouich, Imen
Nasr, Nouha
Ben Ghozlen, Hajer
Mustapha, Nadia
Ghedira, Kamel
Chekir-Ghedira, Leila
author_facet Boubaker, Jihed
Mokdad Bzeouich, Imen
Nasr, Nouha
Ben Ghozlen, Hajer
Mustapha, Nadia
Ghedira, Kamel
Chekir-Ghedira, Leila
author_sort Boubaker, Jihed
collection PubMed
description BACKGROUND: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated. METHODS: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm. RESULTS: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells. CONCLUSION: The tested extracts have a significant biological effect which may be due to their bioactive compounds.
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spelling pubmed-45560502015-09-02 Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma Boubaker, Jihed Mokdad Bzeouich, Imen Nasr, Nouha Ben Ghozlen, Hajer Mustapha, Nadia Ghedira, Kamel Chekir-Ghedira, Leila BMC Complement Altern Med Research Article BACKGROUND: Here, phytochemical profile of Nitraria retusa (N. Retusa) leaf extracts was identified and their ability to induce apoptosis and inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma was evaluated. METHODS: The Apoptosis was evidenced by investigating DNA fragmentation, and Acridine orange/ethidium bromide staining. Amounts of melanin and tyrosinase were measured spectrophotometrically at 475 nm. RESULTS: Extracts from Nitraria retusa exhibited significant anti-proliferative activity after 48 h of incubation. Our result was confirmed by ladder DNA fragmentation profile. All extracts showed also the ability to enhance melanogenesis and tyrosinase activity of B16F10 melanoma cells. CONCLUSION: The tested extracts have a significant biological effect which may be due to their bioactive compounds. BioMed Central 2015-09-02 /pmc/articles/PMC4556050/ /pubmed/26329604 http://dx.doi.org/10.1186/s12906-015-0743-z Text en © Boubaker et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Boubaker, Jihed
Mokdad Bzeouich, Imen
Nasr, Nouha
Ben Ghozlen, Hajer
Mustapha, Nadia
Ghedira, Kamel
Chekir-Ghedira, Leila
Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title_full Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title_fullStr Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title_full_unstemmed Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title_short Phytochemical capacity of Nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of B16F10 melanoma
title_sort phytochemical capacity of nitraria retusa leaves extracts inhibiting growth of melanoma cells and enhancing melanogenesis of b16f10 melanoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556050/
https://www.ncbi.nlm.nih.gov/pubmed/26329604
http://dx.doi.org/10.1186/s12906-015-0743-z
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