Cargando…
Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults
BACKGROUND: Recent publications have reported contradictory data regarding mitochondrial DNA (mtDNA) variation and its association with body mass index. The aim of the present study was to compare the frequencies of mtDNA haplogroups as well as control region (CR) polymorphisms of obese juveniles (n...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556186/ https://www.ncbi.nlm.nih.gov/pubmed/26322975 http://dx.doi.org/10.1371/journal.pone.0135622 |
_version_ | 1782388313727959040 |
---|---|
author | Ebner, Sabine Mangge, Harald Langhof, Helmut Halle, Martin Siegrist, Monika Aigner, Elmar Paulmichl, Katharina Paulweber, Bernhard Datz, Christian Sperl, Wolfgang Kofler, Barbara Weghuber, Daniel |
author_facet | Ebner, Sabine Mangge, Harald Langhof, Helmut Halle, Martin Siegrist, Monika Aigner, Elmar Paulmichl, Katharina Paulweber, Bernhard Datz, Christian Sperl, Wolfgang Kofler, Barbara Weghuber, Daniel |
author_sort | Ebner, Sabine |
collection | PubMed |
description | BACKGROUND: Recent publications have reported contradictory data regarding mitochondrial DNA (mtDNA) variation and its association with body mass index. The aim of the present study was to compare the frequencies of mtDNA haplogroups as well as control region (CR) polymorphisms of obese juveniles (n = 248) and obese adults (n = 1003) versus normal weight controls (n(juvenile) = 266, n(adults) = 595) in a well-defined, ethnically homogenous, age-matched comparative cohort of Austrian Caucasians. METHODOLOGY AND PRINCIPAL FINDINGS: Using SNP analysis and DNA sequencing, we identified the nine major European mitochondrial haplogroups and CR polymorphisms. Of these, only the T haplogroup frequency was increased in the juvenile obese cohort versus the control subjects [11.7% in obese vs. 6.4% in controls], although statistical significance was lost after adjustment for sex and age. Similar data were observed in a local adult cohort, in which haplogroup T was found at a significantly higher frequency in the overweight and obese subjects than in the normal weight group [9.7% vs. 6.2%, p = 0.012, adjusted for sex and age]. When all obese subjects were considered together, the difference in the frequency of haplogroup T was even more clearly seen [10.1% vs. 6.3%, p = 0.002, OR (95% CI) 1.71 (1.2–2.4), adjusted for sex and age]. The frequencies of the T haplogroup-linked CR polymorphisms C16294T and the C16296T were found to be elevated in both the juvenile and the adult obese cohort compared to the controls. Nevertheless, no mtDNA haplogroup or CR polymorphism was robustly associated with any of several investigated metabolic and cardiovascular parameters (e.g., blood pressure, blood glucose concentration, triglycerides, cholesterol) in all obese subjects. CONCLUSIONS AND SIGNIFICANCE: By investigation of this large ethnically and geographically homogenous cohort of Middle European Caucasians, only mtDNA haplogroup T was identified as an obesity risk factor. |
format | Online Article Text |
id | pubmed-4556186 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45561862015-09-10 Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults Ebner, Sabine Mangge, Harald Langhof, Helmut Halle, Martin Siegrist, Monika Aigner, Elmar Paulmichl, Katharina Paulweber, Bernhard Datz, Christian Sperl, Wolfgang Kofler, Barbara Weghuber, Daniel PLoS One Research Article BACKGROUND: Recent publications have reported contradictory data regarding mitochondrial DNA (mtDNA) variation and its association with body mass index. The aim of the present study was to compare the frequencies of mtDNA haplogroups as well as control region (CR) polymorphisms of obese juveniles (n = 248) and obese adults (n = 1003) versus normal weight controls (n(juvenile) = 266, n(adults) = 595) in a well-defined, ethnically homogenous, age-matched comparative cohort of Austrian Caucasians. METHODOLOGY AND PRINCIPAL FINDINGS: Using SNP analysis and DNA sequencing, we identified the nine major European mitochondrial haplogroups and CR polymorphisms. Of these, only the T haplogroup frequency was increased in the juvenile obese cohort versus the control subjects [11.7% in obese vs. 6.4% in controls], although statistical significance was lost after adjustment for sex and age. Similar data were observed in a local adult cohort, in which haplogroup T was found at a significantly higher frequency in the overweight and obese subjects than in the normal weight group [9.7% vs. 6.2%, p = 0.012, adjusted for sex and age]. When all obese subjects were considered together, the difference in the frequency of haplogroup T was even more clearly seen [10.1% vs. 6.3%, p = 0.002, OR (95% CI) 1.71 (1.2–2.4), adjusted for sex and age]. The frequencies of the T haplogroup-linked CR polymorphisms C16294T and the C16296T were found to be elevated in both the juvenile and the adult obese cohort compared to the controls. Nevertheless, no mtDNA haplogroup or CR polymorphism was robustly associated with any of several investigated metabolic and cardiovascular parameters (e.g., blood pressure, blood glucose concentration, triglycerides, cholesterol) in all obese subjects. CONCLUSIONS AND SIGNIFICANCE: By investigation of this large ethnically and geographically homogenous cohort of Middle European Caucasians, only mtDNA haplogroup T was identified as an obesity risk factor. Public Library of Science 2015-08-31 /pmc/articles/PMC4556186/ /pubmed/26322975 http://dx.doi.org/10.1371/journal.pone.0135622 Text en © 2015 Ebner et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Ebner, Sabine Mangge, Harald Langhof, Helmut Halle, Martin Siegrist, Monika Aigner, Elmar Paulmichl, Katharina Paulweber, Bernhard Datz, Christian Sperl, Wolfgang Kofler, Barbara Weghuber, Daniel Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title | Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title_full | Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title_fullStr | Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title_full_unstemmed | Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title_short | Mitochondrial Haplogroup T Is Associated with Obesity in Austrian Juveniles and Adults |
title_sort | mitochondrial haplogroup t is associated with obesity in austrian juveniles and adults |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556186/ https://www.ncbi.nlm.nih.gov/pubmed/26322975 http://dx.doi.org/10.1371/journal.pone.0135622 |
work_keys_str_mv | AT ebnersabine mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT manggeharald mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT langhofhelmut mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT hallemartin mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT siegristmonika mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT aignerelmar mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT paulmichlkatharina mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT paulweberbernhard mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT datzchristian mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT sperlwolfgang mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT koflerbarbara mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults AT weghuberdaniel mitochondrialhaplogrouptisassociatedwithobesityinaustrianjuvenilesandadults |