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Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo

We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cult...

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Autores principales: Park, Jin Sung, Lee, Hyun Jae, Lee, Dong Yeong, Jo, Ho Seung, Jeong, Jin Hoon, Kim, Dong Hee, Nam, Dae Cheol, Lee, Choong Jae, Hwang, Sun-Chul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Applied Pharmacology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556204/
https://www.ncbi.nlm.nih.gov/pubmed/26336584
http://dx.doi.org/10.4062/biomolther.2015.045
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author Park, Jin Sung
Lee, Hyun Jae
Lee, Dong Yeong
Jo, Ho Seung
Jeong, Jin Hoon
Kim, Dong Hee
Nam, Dae Cheol
Lee, Choong Jae
Hwang, Sun-Chul
author_facet Park, Jin Sung
Lee, Hyun Jae
Lee, Dong Yeong
Jo, Ho Seung
Jeong, Jin Hoon
Kim, Dong Hee
Nam, Dae Cheol
Lee, Choong Jae
Hwang, Sun-Chul
author_sort Park, Jin Sung
collection PubMed
description We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes.
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spelling pubmed-45562042015-09-02 Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo Park, Jin Sung Lee, Hyun Jae Lee, Dong Yeong Jo, Ho Seung Jeong, Jin Hoon Kim, Dong Hee Nam, Dae Cheol Lee, Choong Jae Hwang, Sun-Chul Biomol Ther (Seoul) Original Article We evaluated the chondroprotective effects of wogonin by investigating its effects on the gene expression and production of matrix metalloproteinase-3 (MMP-3) in primary cultured rabbit articular chondrocytes, as well as on production of MMP-3 in the rat knee. Rabbit articular chondrocytes were cultured in a monolayer, and RT-PCR was used to measure interleukin-1β (IL-1β)-induced expression of MMP-3, MMP-1, MMP-13, a disintegrin and metalloproteinase with thrombospondin motifs-4 (ADAMTS-4), and type II collagen. In rabbit articular chondrocytes, the effects of wogonin on IL-1β-induced production and proteolytic activity of MMP-3 were investigated using western blot analysis and casein zymography, respectively. The effect of wogonin on MMP-3 protein production was also examined in vivo. In rabbit articular chondrocytes, wogonin inhibited the expression of MMP-3, MMP-1, MMP-13, and ADAMTS-4, but increased expression of type II collagen. Furthermore, wogonin inhibited the production and proteolytic activity of MMP-3 in vitro, and inhibited production of MMP-3 protein in vivo. These results suggest that wogonin can regulate the gene expression and production of MMP-3, by directly acting on articular chondrocytes. The Korean Society of Applied Pharmacology 2015-09 2015-09-01 /pmc/articles/PMC4556204/ /pubmed/26336584 http://dx.doi.org/10.4062/biomolther.2015.045 Text en Copyright © 2015, The Korean Society of Applied Pharmacology http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Park, Jin Sung
Lee, Hyun Jae
Lee, Dong Yeong
Jo, Ho Seung
Jeong, Jin Hoon
Kim, Dong Hee
Nam, Dae Cheol
Lee, Choong Jae
Hwang, Sun-Chul
Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title_full Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title_fullStr Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title_full_unstemmed Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title_short Chondroprotective Effects of Wogonin in Experimental Models of Osteoarthritis in vitro and in vivo
title_sort chondroprotective effects of wogonin in experimental models of osteoarthritis in vitro and in vivo
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556204/
https://www.ncbi.nlm.nih.gov/pubmed/26336584
http://dx.doi.org/10.4062/biomolther.2015.045
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