Cargando…

A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen

PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding...

Descripción completa

Detalles Bibliográficos
Autores principales: Nguyen, Tan Tai, Bea, Sun Oh, Kim, Dong Min, Yoon, Won Jung, Park, Jin-Won, An, Seong Soo A, Ju, Heongkyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556302/
https://www.ncbi.nlm.nih.gov/pubmed/26347331
http://dx.doi.org/10.2147/IJN.S88963
_version_ 1782388330663510016
author Nguyen, Tan Tai
Bea, Sun Oh
Kim, Dong Min
Yoon, Won Jung
Park, Jin-Won
An, Seong Soo A
Ju, Heongkyu
author_facet Nguyen, Tan Tai
Bea, Sun Oh
Kim, Dong Min
Yoon, Won Jung
Park, Jin-Won
An, Seong Soo A
Ju, Heongkyu
author_sort Nguyen, Tan Tai
collection PubMed
description PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg. RESULTS: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability. CONCLUSION: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases.
format Online
Article
Text
id pubmed-4556302
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Dove Medical Press
record_format MEDLINE/PubMed
spelling pubmed-45563022015-09-04 A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen Nguyen, Tan Tai Bea, Sun Oh Kim, Dong Min Yoon, Won Jung Park, Jin-Won An, Seong Soo A Ju, Heongkyu Int J Nanomedicine Original Research PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg. RESULTS: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability. CONCLUSION: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases. Dove Medical Press 2015-08-27 /pmc/articles/PMC4556302/ /pubmed/26347331 http://dx.doi.org/10.2147/IJN.S88963 Text en © 2015 Nguyen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Nguyen, Tan Tai
Bea, Sun Oh
Kim, Dong Min
Yoon, Won Jung
Park, Jin-Won
An, Seong Soo A
Ju, Heongkyu
A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_full A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_fullStr A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_full_unstemmed A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_short A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
title_sort regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556302/
https://www.ncbi.nlm.nih.gov/pubmed/26347331
http://dx.doi.org/10.2147/IJN.S88963
work_keys_str_mv AT nguyentantai aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT beasunoh aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT kimdongmin aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT yoonwonjung aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT parkjinwon aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT anseongsooa aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT juheongkyu aregenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT nguyentantai regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT beasunoh regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT kimdongmin regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT yoonwonjung regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT parkjinwon regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT anseongsooa regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen
AT juheongkyu regenerativelabelfreefiberopticsensorusingsurfaceplasmonresonanceforclinicaldiagnosisoffibrinogen