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A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen
PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556302/ https://www.ncbi.nlm.nih.gov/pubmed/26347331 http://dx.doi.org/10.2147/IJN.S88963 |
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author | Nguyen, Tan Tai Bea, Sun Oh Kim, Dong Min Yoon, Won Jung Park, Jin-Won An, Seong Soo A Ju, Heongkyu |
author_facet | Nguyen, Tan Tai Bea, Sun Oh Kim, Dong Min Yoon, Won Jung Park, Jin-Won An, Seong Soo A Ju, Heongkyu |
author_sort | Nguyen, Tan Tai |
collection | PubMed |
description | PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg. RESULTS: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability. CONCLUSION: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases. |
format | Online Article Text |
id | pubmed-4556302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45563022015-09-04 A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen Nguyen, Tan Tai Bea, Sun Oh Kim, Dong Min Yoon, Won Jung Park, Jin-Won An, Seong Soo A Ju, Heongkyu Int J Nanomedicine Original Research PURPOSE: We present the regenerative label-free fiber optical biosensor that exploits surface plasmon resonance for quantitative detection of fibrinogen (Fbg) extracted from human blood plasma. MATERIALS AND METHODS: The sensor head was made up of a multimode optical fiber with its polymer cladding replaced by metal composite of nanometer thickness made of silver, aluminum, and nickel. The Ni layer coated allowed a direct immobilization of histidine-tagged peptide (HP) on its metal surface without an additional cross-linker in between. On the coated HP layer, immunoglobulin G was then immobilized for specific capturing of Fbg. RESULTS: We demonstrated a real-time quantitative detection of Fbg concentrations with limit of detection of ~10 ng/mL. The fact that the HP layer could be removed by imidazole with acid also permitted us to demonstrate the regeneration of the outermost metal surface of the sensor head for the sensor reusability. CONCLUSION: The sensor detection limit was estimated to be ~10 pM, which was believed to be sensitive enough for detecting Fbg during the clinical diagnosis of cardiovascular diseases, myocardial infarction, strokes, and Alzheimer’s diseases. Dove Medical Press 2015-08-27 /pmc/articles/PMC4556302/ /pubmed/26347331 http://dx.doi.org/10.2147/IJN.S88963 Text en © 2015 Nguyen et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Nguyen, Tan Tai Bea, Sun Oh Kim, Dong Min Yoon, Won Jung Park, Jin-Won An, Seong Soo A Ju, Heongkyu A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title | A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title_full | A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title_fullStr | A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title_full_unstemmed | A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title_short | A regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
title_sort | regenerative label-free fiber optic sensor using surface plasmon resonance for clinical diagnosis of fibrinogen |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556302/ https://www.ncbi.nlm.nih.gov/pubmed/26347331 http://dx.doi.org/10.2147/IJN.S88963 |
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