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Exploiting single-molecule transcript sequencing for eukaryotic gene prediction

We develop a method to predict and validate gene models using PacBio single-molecule, real-time (SMRT) cDNA reads. Ninety-eight percent of full-insert SMRT reads span complete open reading frames. Gene model validation using SMRT reads is developed as automated process. Optimized training and predic...

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Detalles Bibliográficos
Autores principales:	Minoche, André E., Dohm, Juliane C., Schneider, Jessica, Holtgräwe, Daniela, Viehöver, Prisca, Montfort, Magda, Rosleff Sörensen, Thomas, Weisshaar, Bernd, Himmelbauer, Heinz
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2015
Materias:	Method
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556409/ https://www.ncbi.nlm.nih.gov/pubmed/26328666 http://dx.doi.org/10.1186/s13059-015-0729-7

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