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Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions

OBJECTIVE(S): Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects...

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Autores principales: Motlagh, Bahman Mansouri, Ahangaran, Nahideh Afzale, Froushani, Seyyed Meysam Abtahi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mashhad University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556760/
https://www.ncbi.nlm.nih.gov/pubmed/26351558
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author Motlagh, Bahman Mansouri
Ahangaran, Nahideh Afzale
Froushani, Seyyed Meysam Abtahi
author_facet Motlagh, Bahman Mansouri
Ahangaran, Nahideh Afzale
Froushani, Seyyed Meysam Abtahi
author_sort Motlagh, Bahman Mansouri
collection PubMed
description OBJECTIVE(S): Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages. MATERIALS AND METHODS: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability. RESULTS: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker’s yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group. CONCLUSION: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.
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spelling pubmed-45567602015-09-08 Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions Motlagh, Bahman Mansouri Ahangaran, Nahideh Afzale Froushani, Seyyed Meysam Abtahi Iran J Basic Med Sci Original Article OBJECTIVE(S): Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages. MATERIALS AND METHODS: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability. RESULTS: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker’s yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group. CONCLUSION: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs. Mashhad University of Medical Sciences 2015-07 /pmc/articles/PMC4556760/ /pubmed/26351558 Text en Copyright: © Iranian Journal of Basic Medical Sciences http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Motlagh, Bahman Mansouri
Ahangaran, Nahideh Afzale
Froushani, Seyyed Meysam Abtahi
Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title_full Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title_fullStr Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title_full_unstemmed Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title_short Calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
title_sort calcitriol modulates the effects of bone marrow-derived mesenchymal stem cells on macrophage functions
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556760/
https://www.ncbi.nlm.nih.gov/pubmed/26351558
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