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PEA-CLARITY: 3D molecular imaging of whole plant organs
Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins...
Autores principales: | Palmer, William M., Martin, Antony P., Flynn, Jamie R., Reed, Stephanie L., White, Rosemary G., Furbank, Robert T., Grof, Christopher P. L. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556961/ https://www.ncbi.nlm.nih.gov/pubmed/26328508 http://dx.doi.org/10.1038/srep13492 |
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