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PEA-CLARITY: 3D molecular imaging of whole plant organs

Here we report the adaptation of the CLARITY technique to plant tissues with addition of enzymatic degradation to improve optical clearing and facilitate antibody probe penetration. Plant-Enzyme-Assisted (PEA)-CLARITY, has allowed deep optical visualisation of stains, expressed fluorescent proteins...

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Detalles Bibliográficos
Autores principales: Palmer, William M., Martin, Antony P., Flynn, Jamie R., Reed, Stephanie L., White, Rosemary G., Furbank, Robert T., Grof, Christopher P. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4556961/
https://www.ncbi.nlm.nih.gov/pubmed/26328508
http://dx.doi.org/10.1038/srep13492

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