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Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum

Penicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on mul...

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Autores principales: Liu, Qin-Ying, Zhou, Tong, Zhao, Yang-Yang, Chen, Li, Gong, Mei-Wei, Xia, Qi-Wen, Ying, Min-Gang, Zheng, Qiu-Hong, Zhang, Qi-Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557002/
https://www.ncbi.nlm.nih.gov/pubmed/26264002
http://dx.doi.org/10.3390/md13084733
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author Liu, Qin-Ying
Zhou, Tong
Zhao, Yang-Yang
Chen, Li
Gong, Mei-Wei
Xia, Qi-Wen
Ying, Min-Gang
Zheng, Qiu-Hong
Zhang, Qi-Qing
author_facet Liu, Qin-Ying
Zhou, Tong
Zhao, Yang-Yang
Chen, Li
Gong, Mei-Wei
Xia, Qi-Wen
Ying, Min-Gang
Zheng, Qiu-Hong
Zhang, Qi-Qing
author_sort Liu, Qin-Ying
collection PubMed
description Penicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on multiple tumor types. Among them, the human malignant melanoma cell A-375 was confirmed to be the most sensitive. Morphologic evaluation, apoptosis rate analysis, Western blot and real-time quantitative PCR (RT-qPCR) results showed penicitrinine A could significantly induce A-375 cell apoptosis by decreasing the expression of Bcl-2 and increasing the expression of Bax. Moreover, we investigated the anti-metastatic effects of penicitrinine A in A-375 cells by wound healing assay, trans-well assay, Western blot and RT-qPCR. The results showed penicitrinine A significantly suppressed metastatic activity of A-375 cells by regulating the expression of MMP-9 and its specific inhibitor TIMP-1. These findings suggested that penicitrinine A might serve as a potential antitumor agent, which could inhibit the proliferation and metastasis of tumor cells.
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spelling pubmed-45570022015-09-03 Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum Liu, Qin-Ying Zhou, Tong Zhao, Yang-Yang Chen, Li Gong, Mei-Wei Xia, Qi-Wen Ying, Min-Gang Zheng, Qiu-Hong Zhang, Qi-Qing Mar Drugs Article Penicitrinine A, a novel alkaloid with a unique spiro skeleton, was isolated from a marine-derived fungus Penicillium citrinum. In this study, the isolation, structure and biosynthetic pathway elucidation of the new compound were described. This new compound showed anti-proliferative activity on multiple tumor types. Among them, the human malignant melanoma cell A-375 was confirmed to be the most sensitive. Morphologic evaluation, apoptosis rate analysis, Western blot and real-time quantitative PCR (RT-qPCR) results showed penicitrinine A could significantly induce A-375 cell apoptosis by decreasing the expression of Bcl-2 and increasing the expression of Bax. Moreover, we investigated the anti-metastatic effects of penicitrinine A in A-375 cells by wound healing assay, trans-well assay, Western blot and RT-qPCR. The results showed penicitrinine A significantly suppressed metastatic activity of A-375 cells by regulating the expression of MMP-9 and its specific inhibitor TIMP-1. These findings suggested that penicitrinine A might serve as a potential antitumor agent, which could inhibit the proliferation and metastasis of tumor cells. MDPI 2015-07-31 /pmc/articles/PMC4557002/ /pubmed/26264002 http://dx.doi.org/10.3390/md13084733 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Qin-Ying
Zhou, Tong
Zhao, Yang-Yang
Chen, Li
Gong, Mei-Wei
Xia, Qi-Wen
Ying, Min-Gang
Zheng, Qiu-Hong
Zhang, Qi-Qing
Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title_full Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title_fullStr Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title_full_unstemmed Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title_short Antitumor Effects and Related Mechanisms of Penicitrinine A, a Novel Alkaloid with a Unique Spiro Skeleton from the Marine Fungus Penicillium citrinum
title_sort antitumor effects and related mechanisms of penicitrinine a, a novel alkaloid with a unique spiro skeleton from the marine fungus penicillium citrinum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557002/
https://www.ncbi.nlm.nih.gov/pubmed/26264002
http://dx.doi.org/10.3390/md13084733
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