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Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts

Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC(50) values for DPPH scaven...

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Autores principales: Paliwal, Chetan, Ghosh, Tonmoy, Bhayani, Khushbu, Maurya, Rahulkumar, Mishra, Sandhya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557027/
https://www.ncbi.nlm.nih.gov/pubmed/26308007
http://dx.doi.org/10.3390/md13085384
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author Paliwal, Chetan
Ghosh, Tonmoy
Bhayani, Khushbu
Maurya, Rahulkumar
Mishra, Sandhya
author_facet Paliwal, Chetan
Ghosh, Tonmoy
Bhayani, Khushbu
Maurya, Rahulkumar
Mishra, Sandhya
author_sort Paliwal, Chetan
collection PubMed
description Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC(50) values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg(−1) DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg(−1) DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM—Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%–65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%–80%.
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spelling pubmed-45570272015-09-03 Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts Paliwal, Chetan Ghosh, Tonmoy Bhayani, Khushbu Maurya, Rahulkumar Mishra, Sandhya Mar Drugs Article Phycobiliprotein-containing water and carotenoid-containing methanolic extracts of three different cyanobacteria, Pseudanabaena sp., Spirulina sp. and Lyngbya sp., were studied for their DPPH scavenging, iso-bolographic studies, and anti-nephrolithe activities. The best EC(50) values for DPPH scavenging were in Lyngbya water (LW, 18.78 ± 1.57 mg·mg(−1) DPPH) and Lyngbya methanol (LM, 59.56 ± 37.38 mg·mg(−1) DPPH) extracts. Iso-bolographic analysis revealed most of the combinations of extracts were antagonistic to each other, although LM—Spirulina methanol (SM) 1:1 had the highest synergistic rate of 86.65%. In vitro digestion studies showed that DPPH scavenging activity was considerably decreased in all extracts except for Pseudanabaena methanol (PM) and LM after the simulated digestion. All of the extracts were effective in reducing the calcium oxalate crystal size by nearly 60%–65% compared to negative control, while PM and Spirulina water (SW) extracts could inhibit both nucleation and aggregation of calcium oxalate by nearly 60%–80%. MDPI 2015-08-20 /pmc/articles/PMC4557027/ /pubmed/26308007 http://dx.doi.org/10.3390/md13085384 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Paliwal, Chetan
Ghosh, Tonmoy
Bhayani, Khushbu
Maurya, Rahulkumar
Mishra, Sandhya
Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title_full Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title_fullStr Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title_full_unstemmed Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title_short Antioxidant, Anti-Nephrolithe Activities and in Vitro Digestibility Studies of Three Different Cyanobacterial Pigment Extracts
title_sort antioxidant, anti-nephrolithe activities and in vitro digestibility studies of three different cyanobacterial pigment extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557027/
https://www.ncbi.nlm.nih.gov/pubmed/26308007
http://dx.doi.org/10.3390/md13085384
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