Cargando…

The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages

Algae contain a number of anti-inflammatory bioactive compounds such as omega-3 polyunsaturated fatty acids (n-3 PUFA) and chlorophyll a, hence as dietary ingredients, their extracts may be effective in chronic inflammation-linked metabolic diseases such as cardiovascular disease. In this study, ant...

Descripción completa

Detalles Bibliográficos
Autores principales: Robertson, Ruairi C., Guihéneuf, Freddy, Bahar, Bojlul, Schmid, Matthias, Stengel, Dagmar B., Fitzgerald, Gerald F., Ross, R. Paul, Stanton, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557028/
https://www.ncbi.nlm.nih.gov/pubmed/26308008
http://dx.doi.org/10.3390/md13085402
_version_ 1782388437115994112
author Robertson, Ruairi C.
Guihéneuf, Freddy
Bahar, Bojlul
Schmid, Matthias
Stengel, Dagmar B.
Fitzgerald, Gerald F.
Ross, R. Paul
Stanton, Catherine
author_facet Robertson, Ruairi C.
Guihéneuf, Freddy
Bahar, Bojlul
Schmid, Matthias
Stengel, Dagmar B.
Fitzgerald, Gerald F.
Ross, R. Paul
Stanton, Catherine
author_sort Robertson, Ruairi C.
collection PubMed
description Algae contain a number of anti-inflammatory bioactive compounds such as omega-3 polyunsaturated fatty acids (n-3 PUFA) and chlorophyll a, hence as dietary ingredients, their extracts may be effective in chronic inflammation-linked metabolic diseases such as cardiovascular disease. In this study, anti-inflammatory potential of lipid extracts from three red seaweeds (Porphyra dioica, Palmaria palmata and Chondrus crispus) and one microalga (Pavlova lutheri) were assessed in lipopolysaccharide (LPS)-stimulated human THP-1 macrophages. Extracts contained 34%–42% total fatty acids as n-3 PUFA and 5%–7% crude extract as pigments, including chlorophyll a, β-carotene and fucoxanthin. Pretreatment of the THP-1 cells with lipid extract from P. palmata inhibited production of the pro-inflammatory cytokines interleukin (IL)-6 (p < 0.05) and IL-8 (p < 0.05) while that of P. lutheri inhibited IL-6 (p < 0.01) production. Quantitative gene expression analysis of a panel of 92 genes linked to inflammatory signaling pathway revealed down-regulation of the expression of 14 pro-inflammatory genes (TLR1, TLR2, TLR4, TLR8, TRAF5, TRAF6, TNFSF18, IL6R, IL23, CCR1, CCR4, CCL17, STAT3, MAP3K1) by the lipid extracts. The lipid extracts effectively inhibited the LPS-induced pro-inflammatory signaling pathways mediated via toll-like receptors, chemokines and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling molecules. These results suggest that lipid extracts from P. lutheri, P. palmata, P. dioica and C. crispus can inhibit LPS-induced inflammatory pathways in human macrophages. Therefore, algal lipid extracts should be further explored as anti-inflammatory ingredients for chronic inflammation-linked metabolic diseases.
format Online
Article
Text
id pubmed-4557028
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-45570282015-09-03 The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages Robertson, Ruairi C. Guihéneuf, Freddy Bahar, Bojlul Schmid, Matthias Stengel, Dagmar B. Fitzgerald, Gerald F. Ross, R. Paul Stanton, Catherine Mar Drugs Article Algae contain a number of anti-inflammatory bioactive compounds such as omega-3 polyunsaturated fatty acids (n-3 PUFA) and chlorophyll a, hence as dietary ingredients, their extracts may be effective in chronic inflammation-linked metabolic diseases such as cardiovascular disease. In this study, anti-inflammatory potential of lipid extracts from three red seaweeds (Porphyra dioica, Palmaria palmata and Chondrus crispus) and one microalga (Pavlova lutheri) were assessed in lipopolysaccharide (LPS)-stimulated human THP-1 macrophages. Extracts contained 34%–42% total fatty acids as n-3 PUFA and 5%–7% crude extract as pigments, including chlorophyll a, β-carotene and fucoxanthin. Pretreatment of the THP-1 cells with lipid extract from P. palmata inhibited production of the pro-inflammatory cytokines interleukin (IL)-6 (p < 0.05) and IL-8 (p < 0.05) while that of P. lutheri inhibited IL-6 (p < 0.01) production. Quantitative gene expression analysis of a panel of 92 genes linked to inflammatory signaling pathway revealed down-regulation of the expression of 14 pro-inflammatory genes (TLR1, TLR2, TLR4, TLR8, TRAF5, TRAF6, TNFSF18, IL6R, IL23, CCR1, CCR4, CCL17, STAT3, MAP3K1) by the lipid extracts. The lipid extracts effectively inhibited the LPS-induced pro-inflammatory signaling pathways mediated via toll-like receptors, chemokines and nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling molecules. These results suggest that lipid extracts from P. lutheri, P. palmata, P. dioica and C. crispus can inhibit LPS-induced inflammatory pathways in human macrophages. Therefore, algal lipid extracts should be further explored as anti-inflammatory ingredients for chronic inflammation-linked metabolic diseases. MDPI 2015-08-20 /pmc/articles/PMC4557028/ /pubmed/26308008 http://dx.doi.org/10.3390/md13085402 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Robertson, Ruairi C.
Guihéneuf, Freddy
Bahar, Bojlul
Schmid, Matthias
Stengel, Dagmar B.
Fitzgerald, Gerald F.
Ross, R. Paul
Stanton, Catherine
The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title_full The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title_fullStr The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title_full_unstemmed The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title_short The Anti-Inflammatory Effect of Algae-Derived Lipid Extracts on Lipopolysaccharide (LPS)-Stimulated Human THP-1 Macrophages
title_sort anti-inflammatory effect of algae-derived lipid extracts on lipopolysaccharide (lps)-stimulated human thp-1 macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557028/
https://www.ncbi.nlm.nih.gov/pubmed/26308008
http://dx.doi.org/10.3390/md13085402
work_keys_str_mv AT robertsonruairic theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT guiheneuffreddy theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT baharbojlul theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT schmidmatthias theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT stengeldagmarb theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT fitzgeraldgeraldf theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT rossrpaul theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT stantoncatherine theantiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT robertsonruairic antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT guiheneuffreddy antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT baharbojlul antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT schmidmatthias antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT stengeldagmarb antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT fitzgeraldgeraldf antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT rossrpaul antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages
AT stantoncatherine antiinflammatoryeffectofalgaederivedlipidextractsonlipopolysaccharidelpsstimulatedhumanthp1macrophages