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The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways

Abscisic acid (ABA) and gibberellic acid (GA) antagonistically regulate many developmental processes and responses to biotic or abiotic stresses in higher plants. However, the molecular mechanism underlying this antagonism is still poorly understood. Here, we show that loss-of-function mutation in r...

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Autores principales: Lin, Qibing, Wu, Fuqing, Sheng, Peike, Zhang, Zhe, Zhang, Xin, Guo, Xiuping, Wang, Jiulin, Cheng, Zhijun, Wang, Jie, Wang, Haiyang, Wan, Jianmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557272/
https://www.ncbi.nlm.nih.gov/pubmed/26272249
http://dx.doi.org/10.1038/ncomms8981
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author Lin, Qibing
Wu, Fuqing
Sheng, Peike
Zhang, Zhe
Zhang, Xin
Guo, Xiuping
Wang, Jiulin
Cheng, Zhijun
Wang, Jie
Wang, Haiyang
Wan, Jianmin
author_facet Lin, Qibing
Wu, Fuqing
Sheng, Peike
Zhang, Zhe
Zhang, Xin
Guo, Xiuping
Wang, Jiulin
Cheng, Zhijun
Wang, Jie
Wang, Haiyang
Wan, Jianmin
author_sort Lin, Qibing
collection PubMed
description Abscisic acid (ABA) and gibberellic acid (GA) antagonistically regulate many developmental processes and responses to biotic or abiotic stresses in higher plants. However, the molecular mechanism underlying this antagonism is still poorly understood. Here, we show that loss-of-function mutation in rice Tiller Enhancer (TE), an activator of the APC/C(TE) complex, causes hypersensitivity and hyposensitivity to ABA and GA, respectively. We find that TE physically interacts with ABA receptor OsPYL/RCARs and promotes their degradation by the proteasome. Genetic analysis also shows OsPYL/RCARs act downstream of TE in mediating ABA responses. Conversely, ABA inhibits APC/C(TE) activity by phosphorylating TE through activating the SNF1-related protein kinases (SnRK2s), which may interrupt the interaction between TE and OsPYL/RCARs and subsequently stabilize OsPYL/RCARs. In contrast, GA can reduce the level of SnRK2s and may promote APC/C(TE)-mediated degradation of OsPYL/RCARs. Thus, we propose that the SnRK2-APC/C(TE) regulatory module represents a regulatory hub underlying the antagonistic action of GA and ABA in plants.
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spelling pubmed-45572722015-09-14 The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways Lin, Qibing Wu, Fuqing Sheng, Peike Zhang, Zhe Zhang, Xin Guo, Xiuping Wang, Jiulin Cheng, Zhijun Wang, Jie Wang, Haiyang Wan, Jianmin Nat Commun Article Abscisic acid (ABA) and gibberellic acid (GA) antagonistically regulate many developmental processes and responses to biotic or abiotic stresses in higher plants. However, the molecular mechanism underlying this antagonism is still poorly understood. Here, we show that loss-of-function mutation in rice Tiller Enhancer (TE), an activator of the APC/C(TE) complex, causes hypersensitivity and hyposensitivity to ABA and GA, respectively. We find that TE physically interacts with ABA receptor OsPYL/RCARs and promotes their degradation by the proteasome. Genetic analysis also shows OsPYL/RCARs act downstream of TE in mediating ABA responses. Conversely, ABA inhibits APC/C(TE) activity by phosphorylating TE through activating the SNF1-related protein kinases (SnRK2s), which may interrupt the interaction between TE and OsPYL/RCARs and subsequently stabilize OsPYL/RCARs. In contrast, GA can reduce the level of SnRK2s and may promote APC/C(TE)-mediated degradation of OsPYL/RCARs. Thus, we propose that the SnRK2-APC/C(TE) regulatory module represents a regulatory hub underlying the antagonistic action of GA and ABA in plants. Nature Pub. Group 2015-08-14 /pmc/articles/PMC4557272/ /pubmed/26272249 http://dx.doi.org/10.1038/ncomms8981 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lin, Qibing
Wu, Fuqing
Sheng, Peike
Zhang, Zhe
Zhang, Xin
Guo, Xiuping
Wang, Jiulin
Cheng, Zhijun
Wang, Jie
Wang, Haiyang
Wan, Jianmin
The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title_full The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title_fullStr The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title_full_unstemmed The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title_short The SnRK2-APC/C(TE) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
title_sort snrk2-apc/c(te) regulatory module mediates the antagonistic action of gibberellic acid and abscisic acid pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557272/
https://www.ncbi.nlm.nih.gov/pubmed/26272249
http://dx.doi.org/10.1038/ncomms8981
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