Beads-free protein immunoprecipitation for a mass spectrometry-based interactome and posttranslational modifications analysis

BACKGROUND: Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical protein IP assay antibodies are conjugated to protein A/G beads requiring large amounts of antibodies, tube transfers and centri...

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Detalles Bibliográficos
Autores principales: Mikula, Michal, Rubel, Tymon, Karczmarski, Jakub, Statkiewicz, Malgorzata, Bomsztyk, Karol, Ostrowski, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557753/
https://www.ncbi.nlm.nih.gov/pubmed/26336360
http://dx.doi.org/10.1186/s12953-015-0079-0

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