A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()

Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agree...

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Detalles Bibliográficos
Autores principales: Hardick, Justin, Sadiq, Sufyan, Perelstein, Elizabeth, Peterson, Stephen, Rothman, Richard, Gaydos, Charlotte A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557781/
https://www.ncbi.nlm.nih.gov/pubmed/24657170
http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.009
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author Hardick, Justin
Sadiq, Sufyan
Perelstein, Elizabeth
Peterson, Stephen
Rothman, Richard
Gaydos, Charlotte A.
author_facet Hardick, Justin
Sadiq, Sufyan
Perelstein, Elizabeth
Peterson, Stephen
Rothman, Richard
Gaydos, Charlotte A.
author_sort Hardick, Justin
collection PubMed
description Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9–100%, and specificity was 99–100%.
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spelling pubmed-45577812015-09-02 A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()() Hardick, Justin Sadiq, Sufyan Perelstein, Elizabeth Peterson, Stephen Rothman, Richard Gaydos, Charlotte A. Diagn Microbiol Infect Dis Article Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9–100%, and specificity was 99–100%. Elsevier Inc. 2014-06 2014-02-24 /pmc/articles/PMC4557781/ /pubmed/24657170 http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.009 Text en Copyright © 2014 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Hardick, Justin
Sadiq, Sufyan
Perelstein, Elizabeth
Peterson, Stephen
Rothman, Richard
Gaydos, Charlotte A.
A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title_full A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title_fullStr A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title_full_unstemmed A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title_short A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR()()()
title_sort case-control study evaluating rt-pcr/esi-ms technology compared to direct fluorescent antibody and xtag rvp pcr()()()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4557781/
https://www.ncbi.nlm.nih.gov/pubmed/24657170
http://dx.doi.org/10.1016/j.diagmicrobio.2014.02.009
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