Cargando…

An Integrated System for Precise Genome Modification in Escherichia coli

We describe an optimized system for the easy, effective, and precise modification of the Escherichia coli genome. Genome changes are introduced first through the integration of a 1.3 kbp Landing Pad consisting of a gene conferring resistance to tetracycline (tetA) or the ability to metabolize the su...

Descripción completa

Detalles Bibliográficos
Autores principales: Tas, Huseyin, Nguyen, Cac T., Patel, Ravish, Kim, Neil H., Kuhlman, Thomas E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558010/
https://www.ncbi.nlm.nih.gov/pubmed/26332675
http://dx.doi.org/10.1371/journal.pone.0136963

Ejemplares similares