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Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni

Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms by which the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that direct...

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Autores principales: Pereira, Thiago A., Syn, Wing-Kin, Machado, Mariana V., Vidigal, Paula V., Resende, Vivian, Voieta, Izabela, Xie, Guanhua, Otoni, Alba, Souza, Márcia M., Santos, Elisângela T., Chan, Isaac S., Trindade, Guilherme V.M., Choi, Steve S., Witek, Rafal P., Pereira, Fausto E., Secor, William E., Andrade, Zilton A., Lambertucci, José Roberto, Diehl, Anna Mae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558314/
https://www.ncbi.nlm.nih.gov/pubmed/26201095
http://dx.doi.org/10.1042/CS20150117
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author Pereira, Thiago A.
Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula V.
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia M.
Santos, Elisângela T.
Chan, Isaac S.
Trindade, Guilherme V.M.
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto E.
Secor, William E.
Andrade, Zilton A.
Lambertucci, José Roberto
Diehl, Anna Mae
author_facet Pereira, Thiago A.
Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula V.
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia M.
Santos, Elisângela T.
Chan, Isaac S.
Trindade, Guilherme V.M.
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto E.
Secor, William E.
Andrade, Zilton A.
Lambertucci, José Roberto
Diehl, Anna Mae
author_sort Pereira, Thiago A.
collection PubMed
description Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms by which the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that directly stimulate liver cells to produce osteopontin (OPN), a pro-fibrogenic protein that stimulates hepatic stellate cells to become myofibroblasts. We also investigated the utility of OPN as a biomarker of fibrosis and/or severity of portal hypertension. Cultured cholangiocytes, Kupffer cells and hepatic stellate cells were treated with soluble egg antigen (SEA); OPN production was quantified by quantitative reverse transcriptase polymerase chain reaction (qRTPCR) and ELISA; cell proliferation was assessed by BrdU (5-bromo-2'-deoxyuridine). Mice were infected with Schistosoma mansoni for 6 or 16 weeks to cause early or advanced fibrosis. Liver OPN was evaluated by qRTPCR and immunohistochemistry (IHC) and correlated with liver fibrosis and serum OPN. Livers from patients with schistosomiasis mansoni (early fibrosis n=15; advanced fibrosis n=72) or healthy adults (n=22) were immunostained for OPN and fibrosis markers. Results were correlated with plasma OPN levels and splenic vein pressures. SEA-induced cholangiocyte proliferation and OPN secretion (P<0.001 compared with controls). Cholangiocytes were OPN (+) in Schistosoma-infected mice and humans. Liver and serum OPN levels correlated with fibrosis stage (mice: r=0.861; human r=0.672, P=0.0001) and myofibroblast accumulation (mice: r=0.800; human: r=0.761, P=0.0001). Numbers of OPN (+) bile ductules strongly correlated with splenic vein pressure (r=0.778; P=0.001). S. mansoni egg antigens stimulate cholangiocyte proliferation and OPN secretion. OPN levels in liver and blood correlate with fibrosis stage and portal hypertension severity.
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spelling pubmed-45583142015-10-23 Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni Pereira, Thiago A. Syn, Wing-Kin Machado, Mariana V. Vidigal, Paula V. Resende, Vivian Voieta, Izabela Xie, Guanhua Otoni, Alba Souza, Márcia M. Santos, Elisângela T. Chan, Isaac S. Trindade, Guilherme V.M. Choi, Steve S. Witek, Rafal P. Pereira, Fausto E. Secor, William E. Andrade, Zilton A. Lambertucci, José Roberto Diehl, Anna Mae Clin Sci (Lond) Original Papers Schistosomiasis is a major cause of portal hypertension worldwide. It associates with portal fibrosis that develops during chronic infection. The mechanisms by which the pathogen evokes these host responses remain unclear. We evaluated the hypothesis that schistosome eggs release factors that directly stimulate liver cells to produce osteopontin (OPN), a pro-fibrogenic protein that stimulates hepatic stellate cells to become myofibroblasts. We also investigated the utility of OPN as a biomarker of fibrosis and/or severity of portal hypertension. Cultured cholangiocytes, Kupffer cells and hepatic stellate cells were treated with soluble egg antigen (SEA); OPN production was quantified by quantitative reverse transcriptase polymerase chain reaction (qRTPCR) and ELISA; cell proliferation was assessed by BrdU (5-bromo-2'-deoxyuridine). Mice were infected with Schistosoma mansoni for 6 or 16 weeks to cause early or advanced fibrosis. Liver OPN was evaluated by qRTPCR and immunohistochemistry (IHC) and correlated with liver fibrosis and serum OPN. Livers from patients with schistosomiasis mansoni (early fibrosis n=15; advanced fibrosis n=72) or healthy adults (n=22) were immunostained for OPN and fibrosis markers. Results were correlated with plasma OPN levels and splenic vein pressures. SEA-induced cholangiocyte proliferation and OPN secretion (P<0.001 compared with controls). Cholangiocytes were OPN (+) in Schistosoma-infected mice and humans. Liver and serum OPN levels correlated with fibrosis stage (mice: r=0.861; human r=0.672, P=0.0001) and myofibroblast accumulation (mice: r=0.800; human: r=0.761, P=0.0001). Numbers of OPN (+) bile ductules strongly correlated with splenic vein pressure (r=0.778; P=0.001). S. mansoni egg antigens stimulate cholangiocyte proliferation and OPN secretion. OPN levels in liver and blood correlate with fibrosis stage and portal hypertension severity. Portland Press Ltd. 2015-09-01 2015-11-01 /pmc/articles/PMC4558314/ /pubmed/26201095 http://dx.doi.org/10.1042/CS20150117 Text en © 2015 Authors; published by Portland Press Limited
spellingShingle Original Papers
Pereira, Thiago A.
Syn, Wing-Kin
Machado, Mariana V.
Vidigal, Paula V.
Resende, Vivian
Voieta, Izabela
Xie, Guanhua
Otoni, Alba
Souza, Márcia M.
Santos, Elisângela T.
Chan, Isaac S.
Trindade, Guilherme V.M.
Choi, Steve S.
Witek, Rafal P.
Pereira, Fausto E.
Secor, William E.
Andrade, Zilton A.
Lambertucci, José Roberto
Diehl, Anna Mae
Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_full Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_fullStr Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_full_unstemmed Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_short Schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
title_sort schistosome-induced cholangiocyte proliferation and osteopontin secretion correlate with fibrosis and portal hypertension in human and murine schistosomiasis mansoni
topic Original Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558314/
https://www.ncbi.nlm.nih.gov/pubmed/26201095
http://dx.doi.org/10.1042/CS20150117
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