KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation

Transcription factor SOX4 has been implicated in skeletal myoblast differentiation through the regulation of Cald1 gene expression; however, the detailed molecular mechanism underlying this process is largely unknown. Here, we demonstrate that SOX4 acetylation at lysine 95 by KAT5 (also known as Tip...

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Autores principales: Jang, S-M, Kim, J-W, Kim, C-H, An, J-H, Johnson, A, Song, P I, Rhee, S, Choi, K-H
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558493/
https://www.ncbi.nlm.nih.gov/pubmed/26291311
http://dx.doi.org/10.1038/cddis.2015.190
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author Jang, S-M
Kim, J-W
Kim, C-H
An, J-H
Johnson, A
Song, P I
Rhee, S
Choi, K-H
author_facet Jang, S-M
Kim, J-W
Kim, C-H
An, J-H
Johnson, A
Song, P I
Rhee, S
Choi, K-H
author_sort Jang, S-M
collection PubMed
description Transcription factor SOX4 has been implicated in skeletal myoblast differentiation through the regulation of Cald1 gene expression; however, the detailed molecular mechanism underlying this process is largely unknown. Here, we demonstrate that SOX4 acetylation at lysine 95 by KAT5 (also known as Tip60) is essential for Cald1 promoter activity at the onset of C2C12 myoblast differentiation. KAT5 chromodomain was found to facilitate SOX4 recruitment to the Cald1 promoter, which is involved in chromatin remodeling at the promoter. Chromatin occupancy analysis of SOX4, KAT5, and HDAC1 indicated that the expression of putative SOX4 target genes during C2C12 myoblast differentiation is specifically regulated by the molecular switching of the co-activator KAT5 and the co-repressor HDAC1 on SOX4 transcriptional activation.
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spelling pubmed-45584932015-09-11 KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation Jang, S-M Kim, J-W Kim, C-H An, J-H Johnson, A Song, P I Rhee, S Choi, K-H Cell Death Dis Original Article Transcription factor SOX4 has been implicated in skeletal myoblast differentiation through the regulation of Cald1 gene expression; however, the detailed molecular mechanism underlying this process is largely unknown. Here, we demonstrate that SOX4 acetylation at lysine 95 by KAT5 (also known as Tip60) is essential for Cald1 promoter activity at the onset of C2C12 myoblast differentiation. KAT5 chromodomain was found to facilitate SOX4 recruitment to the Cald1 promoter, which is involved in chromatin remodeling at the promoter. Chromatin occupancy analysis of SOX4, KAT5, and HDAC1 indicated that the expression of putative SOX4 target genes during C2C12 myoblast differentiation is specifically regulated by the molecular switching of the co-activator KAT5 and the co-repressor HDAC1 on SOX4 transcriptional activation. Nature Publishing Group 2015-08 2015-08-20 /pmc/articles/PMC4558493/ /pubmed/26291311 http://dx.doi.org/10.1038/cddis.2015.190 Text en Copyright © 2015 Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Original Article
Jang, S-M
Kim, J-W
Kim, C-H
An, J-H
Johnson, A
Song, P I
Rhee, S
Choi, K-H
KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title_full KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title_fullStr KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title_full_unstemmed KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title_short KAT5-mediated SOX4 acetylation orchestrates chromatin remodeling during myoblast differentiation
title_sort kat5-mediated sox4 acetylation orchestrates chromatin remodeling during myoblast differentiation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558493/
https://www.ncbi.nlm.nih.gov/pubmed/26291311
http://dx.doi.org/10.1038/cddis.2015.190
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