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Droplet Digital Enzyme-Linked Oligonucleotide Hybridization Assay for Absolute RNA Quantification

We present a continuous-flow droplet-based digital Enzyme-Linked Oligonucleotide Hybridization Assay (droplet digital ELOHA) for sensitive detection and absolute quantification of RNA molecules. Droplet digital ELOHA incorporates direct hybridization and single enzyme reaction via the formation of s...

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Detalles Bibliográficos
Autores principales: Guan, Weihua, Chen, Liben, Rane, Tushar D., Wang, Tza-Huei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558716/
https://www.ncbi.nlm.nih.gov/pubmed/26333806
http://dx.doi.org/10.1038/srep13795