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Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis

BACKGROUND: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japoni...

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Autores principales: Zhang, Yuanbin, Zhao, Jing, Wang, Xinye, Xu, Xindong, Pan, Weiqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558877/
https://www.ncbi.nlm.nih.gov/pubmed/26338369
http://dx.doi.org/10.1186/s13071-015-1048-2
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author Zhang, Yuanbin
Zhao, Jing
Wang, Xinye
Xu, Xindong
Pan, Weiqing
author_facet Zhang, Yuanbin
Zhao, Jing
Wang, Xinye
Xu, Xindong
Pan, Weiqing
author_sort Zhang, Yuanbin
collection PubMed
description BACKGROUND: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits. METHODS: The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum. RESULTS: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodies against SjSP-13 and SjSP-216 at 3 weeks post-infection. However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point. We recorded 100 % diagnostic sensitivity and specificity of SjSP-216-based ELISA in both infected mice and rabbits, 3 weeks after infection. CONCLUSIONS: This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1048-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-45588772015-09-04 Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis Zhang, Yuanbin Zhao, Jing Wang, Xinye Xu, Xindong Pan, Weiqing Parasit Vectors Research BACKGROUND: Early diagnosis of schistosomiasis, prior to egg laying, would enable earlier treatment and help interrupt the transmission cycle of the parasite and the progress of the disease. Previously we identified six novel antigens with potential as diagnostic markers for human Schistosoma japonicum infections. In this study, we evaluated these antigens as candidate biomarkers for the early diagnosis of schistosomiasis in mice and rabbits. METHODS: The transcriptional profiles of the six antigens (SjSP-13, SjSP-23, SjSP-160, SjSP-164, SjSP-189 and SjSP-216) at different developmental stages were analyzed by quantitative PCR. The recombinant proteins were expressed in E. coli and purified with nickel chelate affinity chromatography. We then developed recombinant protein-based ELISA kits to analyze the kinetics of antigen-specific antibodies during the course of infection in mice and rabbits. The early diagnostic validity of the candidate SjSP-216 was further evaluated in mice and rabbits infected with S. japonicum. RESULTS: Of the six antigens, SjSP-13, SjSP-160 and SjSP-216 were highly expressed in 21-day old young worms, while SjSP-23, SjSP-164 and SjSP-189 were highly expressed in eggs. In the mouse model, we detected a significant increase in antibodies against SjSP-13 and SjSP-216 at 3 weeks post-infection. However, in the rabbit model, only anti-SjSP-216 antibody showed a significant increase at this time point. We recorded 100 % diagnostic sensitivity and specificity of SjSP-216-based ELISA in both infected mice and rabbits, 3 weeks after infection. CONCLUSIONS: This study strongly suggests that SjSP-216, a highly expressed gene in the young worms, could serve as a potential biomarker for the early immunodiagnosis of S. japonicum infections in vertebrate hosts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-015-1048-2) contains supplementary material, which is available to authorized users. BioMed Central 2015-09-04 /pmc/articles/PMC4558877/ /pubmed/26338369 http://dx.doi.org/10.1186/s13071-015-1048-2 Text en © Zhang et al. 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Zhang, Yuanbin
Zhao, Jing
Wang, Xinye
Xu, Xindong
Pan, Weiqing
Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title_full Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title_fullStr Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title_full_unstemmed Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title_short Evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
title_sort evaluation of six novel antigens as potential biomarkers for the early immunodiagnosis of schistosomiasis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4558877/
https://www.ncbi.nlm.nih.gov/pubmed/26338369
http://dx.doi.org/10.1186/s13071-015-1048-2
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