Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome

The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-pola...

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Autores principales: Haggarty, Jennifer, Oppermann, Madalina, Dalby, Matthew J., Burchmore, Richard J., Cook, Ken, Weidt, Stefan, Burgess, Karl E. V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559102/
https://www.ncbi.nlm.nih.gov/pubmed/26366140
http://dx.doi.org/10.1007/s11306-014-0770-7
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author Haggarty, Jennifer
Oppermann, Madalina
Dalby, Matthew J.
Burchmore, Richard J.
Cook, Ken
Weidt, Stefan
Burgess, Karl E. V.
author_facet Haggarty, Jennifer
Oppermann, Madalina
Dalby, Matthew J.
Burchmore, Richard J.
Cook, Ken
Weidt, Stefan
Burgess, Karl E. V.
author_sort Haggarty, Jennifer
collection PubMed
description The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-014-0770-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-45591022015-09-09 Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome Haggarty, Jennifer Oppermann, Madalina Dalby, Matthew J. Burchmore, Richard J. Cook, Ken Weidt, Stefan Burgess, Karl E. V. Metabolomics Short Communication The serial coupling of a reversed-phase liquid chromatography (RPLC) column to a hydrophilic interaction liquid chromatography (HILIC) column has been developed in recent years for the detection of polar and nonpolar metabolites. TCA intermediates, bile acid standards and numerous polar and non-polar metabolites extracted from beer were analysed using a combined RPLC/HILIC method. Non-polar metabolites were retained by the RPLC column. Polar metabolites not retained by the RPLC column were retained and separated by the HILIC column. The results from this study validate this simple yet powerful metabolomics approach. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-014-0770-7) contains supplementary material, which is available to authorized users. Springer US 2015-01-11 2015 /pmc/articles/PMC4559102/ /pubmed/26366140 http://dx.doi.org/10.1007/s11306-014-0770-7 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Short Communication
Haggarty, Jennifer
Oppermann, Madalina
Dalby, Matthew J.
Burchmore, Richard J.
Cook, Ken
Weidt, Stefan
Burgess, Karl E. V.
Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title_full Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title_fullStr Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title_full_unstemmed Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title_short Serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
title_sort serially coupling hydrophobic interaction and reversed-phase chromatography with simultaneous gradients provides greater coverage of the metabolome
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559102/
https://www.ncbi.nlm.nih.gov/pubmed/26366140
http://dx.doi.org/10.1007/s11306-014-0770-7
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