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Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel

BACKGROUND: Chloroquine has been administered to the soldiers of the Republic of Korea as prophylaxis against vivax malaria. Recent increase in the number of chloroquine-resistant parasites has raised concern over the chemoprophylaxis and treatment of vivax malaria. METHODS: To monitor the developme...

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Autores principales: Chung, Dong-Il, Jeong, Sookwan, Dinzouna-Boutamba, Sylvatrie-Danne, Yang, Hye-Won, Yeo, Sang-Geon, Hong, Yeonchul, Goo, Youn-Kyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559299/
https://www.ncbi.nlm.nih.gov/pubmed/26337571
http://dx.doi.org/10.1186/s12936-015-0845-6
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author Chung, Dong-Il
Jeong, Sookwan
Dinzouna-Boutamba, Sylvatrie-Danne
Yang, Hye-Won
Yeo, Sang-Geon
Hong, Yeonchul
Goo, Youn-Kyoung
author_facet Chung, Dong-Il
Jeong, Sookwan
Dinzouna-Boutamba, Sylvatrie-Danne
Yang, Hye-Won
Yeo, Sang-Geon
Hong, Yeonchul
Goo, Youn-Kyoung
author_sort Chung, Dong-Il
collection PubMed
description BACKGROUND: Chloroquine has been administered to the soldiers of the Republic of Korea as prophylaxis against vivax malaria. Recent increase in the number of chloroquine-resistant parasites has raised concern over the chemoprophylaxis and treatment of vivax malaria. METHODS: To monitor the development of chloroquine-resistant parasites in the Republic of Korea, analyses of single nucleotide polymorphisms (SNPs) of pvmdr1 and microsatellite markers were performed using samples collected from 55 South Korean soldiers infected with Plasmodium vivax. RESULTS: Four SNPs, F1076L, T529, E1233, and S1358, were identified. Among these, S1358 was detected for the first time in Korea. The microsatellite-based study revealed higher genetic diversity in samples collected in 2012 than in 2011. CONCLUSIONS: Taken together, the results indicate that P. vivax with a newly identified SNP of pvmdr1 has been introduced into the Korean P. vivax population. Therefore, continuous monitoring for chloroquine-resistant parasites is required for controlling vivax malaria in the Republic of Korea.
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spelling pubmed-45592992015-09-04 Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel Chung, Dong-Il Jeong, Sookwan Dinzouna-Boutamba, Sylvatrie-Danne Yang, Hye-Won Yeo, Sang-Geon Hong, Yeonchul Goo, Youn-Kyoung Malar J Research BACKGROUND: Chloroquine has been administered to the soldiers of the Republic of Korea as prophylaxis against vivax malaria. Recent increase in the number of chloroquine-resistant parasites has raised concern over the chemoprophylaxis and treatment of vivax malaria. METHODS: To monitor the development of chloroquine-resistant parasites in the Republic of Korea, analyses of single nucleotide polymorphisms (SNPs) of pvmdr1 and microsatellite markers were performed using samples collected from 55 South Korean soldiers infected with Plasmodium vivax. RESULTS: Four SNPs, F1076L, T529, E1233, and S1358, were identified. Among these, S1358 was detected for the first time in Korea. The microsatellite-based study revealed higher genetic diversity in samples collected in 2012 than in 2011. CONCLUSIONS: Taken together, the results indicate that P. vivax with a newly identified SNP of pvmdr1 has been introduced into the Korean P. vivax population. Therefore, continuous monitoring for chloroquine-resistant parasites is required for controlling vivax malaria in the Republic of Korea. BioMed Central 2015-09-04 /pmc/articles/PMC4559299/ /pubmed/26337571 http://dx.doi.org/10.1186/s12936-015-0845-6 Text en © Chung et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Chung, Dong-Il
Jeong, Sookwan
Dinzouna-Boutamba, Sylvatrie-Danne
Yang, Hye-Won
Yeo, Sang-Geon
Hong, Yeonchul
Goo, Youn-Kyoung
Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title_full Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title_fullStr Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title_full_unstemmed Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title_short Evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in Plasmodiumvivax isolates from Republic of Korea military personnel
title_sort evaluation of single nucleotide polymorphisms of pvmdr1 and microsatellite genotype in plasmodiumvivax isolates from republic of korea military personnel
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559299/
https://www.ncbi.nlm.nih.gov/pubmed/26337571
http://dx.doi.org/10.1186/s12936-015-0845-6
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