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Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria
Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compa...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559671/ https://www.ncbi.nlm.nih.gov/pubmed/26337101 http://dx.doi.org/10.1038/srep13517 |
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author | Garrido, Daniel Ruiz-Moyano, Santiago Lemay, Danielle G. Sela, David A. German, J. Bruce Mills, David A. |
author_facet | Garrido, Daniel Ruiz-Moyano, Santiago Lemay, Danielle G. Sela, David A. German, J. Bruce Mills, David A. |
author_sort | Garrido, Daniel |
collection | PubMed |
description | Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria. |
format | Online Article Text |
id | pubmed-4559671 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45596712015-09-11 Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria Garrido, Daniel Ruiz-Moyano, Santiago Lemay, Danielle G. Sela, David A. German, J. Bruce Mills, David A. Sci Rep Article Breast milk enhances the predominance of Bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (HMO). Here we screened infant-gut isolates of Bifidobacterium longum subsp. infantis and Bifidobacterium bifidum using individual HMO, and compared the global transcriptomes of representative isolates on major HMO by RNA-seq. While B. infantis displayed homogeneous HMO-utilization patterns, B. bifidum were more diverse and some strains did not use fucosyllactose (FL) or sialyllactose (SL). Transcriptomes of B. bifidum SC555 and B. infantis ATCC 15697 showed that utilization of pooled HMO is similar to neutral HMO, while transcriptomes for growth on FL were more similar to lactose than HMO in B. bifidum. Genes linked to HMO-utilization were upregulated by neutral HMO and SL, but not by FL in both species. In contrast, FL induced the expression of alternative gene clusters in B. infantis. Results also suggest that B. bifidum SC555 does not utilize fucose or sialic acid from HMO. Surprisingly, expression of orthologous genes differed between both bifidobacteria even when grown on identical substrates. This study highlights two major strategies found in Bifidobacterium species to process HMO, and presents detailed information on the close relationship between HMO and infant-gut bifidobacteria. Nature Publishing Group 2015-09-04 /pmc/articles/PMC4559671/ /pubmed/26337101 http://dx.doi.org/10.1038/srep13517 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Garrido, Daniel Ruiz-Moyano, Santiago Lemay, Danielle G. Sela, David A. German, J. Bruce Mills, David A. Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title_full | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title_fullStr | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title_full_unstemmed | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title_short | Comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
title_sort | comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559671/ https://www.ncbi.nlm.nih.gov/pubmed/26337101 http://dx.doi.org/10.1038/srep13517 |
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