Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence

We describe two-step fluorescence microscopy, a new approach to non-linear imaging based on positive reversible photoswitchable fluorescent probes. The protein Padron approximates ideal two-step fluorescent behaviour: it equilibrates to an inactive state, converts to an active state under blue light...

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Detalles Bibliográficos
Autores principales: Ingaramo, Maria, York, Andrew G., Andrade, Eric J., Rainey, Kristin, Patterson, George H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559865/
https://www.ncbi.nlm.nih.gov/pubmed/26333365
http://dx.doi.org/10.1038/ncomms9184

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559865/
https://www.ncbi.nlm.nih.gov/pubmed/26333365
http://dx.doi.org/10.1038/ncomms9184

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