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Two-photon-like microscopy with orders-of-magnitude lower illumination intensity via two-step fluorescence
We describe two-step fluorescence microscopy, a new approach to non-linear imaging based on positive reversible photoswitchable fluorescent probes. The protein Padron approximates ideal two-step fluorescent behaviour: it equilibrates to an inactive state, converts to an active state under blue light...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Pub. Group
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4559865/ https://www.ncbi.nlm.nih.gov/pubmed/26333365 http://dx.doi.org/10.1038/ncomms9184 |