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Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells

Lead sulfide (PbS) quantum dots (QDs) have been applied in the biomedical area because they offer an excellent platform for theragnostic applications. In order to comprehensively evaluate the biocompatibility of PbS QDs in human cells, we analyzed the exosomes secreted from cells because exosomes ar...

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Autores principales: Kim, Jung-Hee, Kim, Hye-Rim, Lee, Bo-Ram, Choi, Eun-Sook, In, Su-Il, Kim, Eunjoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560511/
https://www.ncbi.nlm.nih.gov/pubmed/26355701
http://dx.doi.org/10.2147/IJN.S89593
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author Kim, Jung-Hee
Kim, Hye-Rim
Lee, Bo-Ram
Choi, Eun-Sook
In, Su-Il
Kim, Eunjoo
author_facet Kim, Jung-Hee
Kim, Hye-Rim
Lee, Bo-Ram
Choi, Eun-Sook
In, Su-Il
Kim, Eunjoo
author_sort Kim, Jung-Hee
collection PubMed
description Lead sulfide (PbS) quantum dots (QDs) have been applied in the biomedical area because they offer an excellent platform for theragnostic applications. In order to comprehensively evaluate the biocompatibility of PbS QDs in human cells, we analyzed the exosomes secreted from cells because exosomes are released during cellular stress to convey signals to other cells and serve as a reservoir of enriched biomarkers. PbS QDs were synthesized and coated with 3-mercaptopropionic acid (MPA) to allow the particles to disperse in water. Exosomes were isolated from HEK293 cells treated with PbS–MPA at concentrations of 0 µg/mL, 5 µg/mL, and 50 µg/mL, and the exosomal expression levels of miRNAs and proteins were analyzed. As a result, five miRNAs and two proteins were proposed as specific exosomal biomarkers for the exposure of HEK293 cells to PbS–MPA. Based on the pathway analysis, the molecular signature of the exosomes suggested that PbS–MPA QDs had carcinogenic activity. The comet assay and expression of molecular markers, such as p53, interleukin (IL)-8, and C-X-C motif chemokine 5, indicated that DNA damage occurred in HEK293 cells following PbS–MPA exposure, which supported the carcinogenic activity of the particles. In addition, there was obvious intensification of miRNA expression signals in the exosomes compared with that of the parent cells, which suggested that exosomal biomarkers could be detected more sensitively than those of whole cellular extracts.
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spelling pubmed-45605112015-09-09 Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells Kim, Jung-Hee Kim, Hye-Rim Lee, Bo-Ram Choi, Eun-Sook In, Su-Il Kim, Eunjoo Int J Nanomedicine Original Research Lead sulfide (PbS) quantum dots (QDs) have been applied in the biomedical area because they offer an excellent platform for theragnostic applications. In order to comprehensively evaluate the biocompatibility of PbS QDs in human cells, we analyzed the exosomes secreted from cells because exosomes are released during cellular stress to convey signals to other cells and serve as a reservoir of enriched biomarkers. PbS QDs were synthesized and coated with 3-mercaptopropionic acid (MPA) to allow the particles to disperse in water. Exosomes were isolated from HEK293 cells treated with PbS–MPA at concentrations of 0 µg/mL, 5 µg/mL, and 50 µg/mL, and the exosomal expression levels of miRNAs and proteins were analyzed. As a result, five miRNAs and two proteins were proposed as specific exosomal biomarkers for the exposure of HEK293 cells to PbS–MPA. Based on the pathway analysis, the molecular signature of the exosomes suggested that PbS–MPA QDs had carcinogenic activity. The comet assay and expression of molecular markers, such as p53, interleukin (IL)-8, and C-X-C motif chemokine 5, indicated that DNA damage occurred in HEK293 cells following PbS–MPA exposure, which supported the carcinogenic activity of the particles. In addition, there was obvious intensification of miRNA expression signals in the exosomes compared with that of the parent cells, which suggested that exosomal biomarkers could be detected more sensitively than those of whole cellular extracts. Dove Medical Press 2015-08-31 /pmc/articles/PMC4560511/ /pubmed/26355701 http://dx.doi.org/10.2147/IJN.S89593 Text en © 2015 Kim et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Kim, Jung-Hee
Kim, Hye-Rim
Lee, Bo-Ram
Choi, Eun-Sook
In, Su-Il
Kim, Eunjoo
Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title_full Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title_fullStr Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title_full_unstemmed Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title_short Carcinogenic activity of PbS quantum dots screened using exosomal biomarkers secreted from HEK293 cells
title_sort carcinogenic activity of pbs quantum dots screened using exosomal biomarkers secreted from hek293 cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560511/
https://www.ncbi.nlm.nih.gov/pubmed/26355701
http://dx.doi.org/10.2147/IJN.S89593
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