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An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering
Current nerve tissue engineering applications are adopting xenogeneic nerve tissue as potential nerve grafts to help aid nerve regeneration. However, there is little literature that describes the exact location, anatomy and physiology of these nerves to highlight their potential as a donor graft. Th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560565/ https://www.ncbi.nlm.nih.gov/pubmed/26200940 http://dx.doi.org/10.1111/joa.12341 |
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author | Zilic, Leyla Garner, Philippa E Yu, Tong Roman, Sabiniano Haycock, John W Wilshaw, Stacy-Paul |
author_facet | Zilic, Leyla Garner, Philippa E Yu, Tong Roman, Sabiniano Haycock, John W Wilshaw, Stacy-Paul |
author_sort | Zilic, Leyla |
collection | PubMed |
description | Current nerve tissue engineering applications are adopting xenogeneic nerve tissue as potential nerve grafts to help aid nerve regeneration. However, there is little literature that describes the exact location, anatomy and physiology of these nerves to highlight their potential as a donor graft. The aim of this study was to identify and characterise the structural and extracellular matrix (ECM) components of porcine peripheral nerves in the hind leg. Methods included the dissection of porcine nerves, localisation, characterisation and quantification of the ECM components and identification of nerve cells. Results showed a noticeable variance between porcine and rat nerve (a commonly studied species) in terms of fascicle number. The study also revealed that when porcine peripheral nerves branch, a decrease in fascicle number and size was evident. Porcine ECM and nerve fascicles were found to be predominately comprised of collagen together with glycosaminoglycans, laminin and fibronectin. Immunolabelling for nerve growth factor receptor p75 also revealed the localisation of Schwann cells around and inside the fascicles. In conclusion, it is shown that porcine peripheral nerves possess a microstructure similar to that found in rat, and is not dissimilar to human. This finding could extend to the suggestion that due to the similarities in anatomy to human nerve, porcine nerves may have utility as a nerve graft providing guidance and support to regenerating axons. |
format | Online Article Text |
id | pubmed-4560565 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley & Sons, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45605652016-09-06 An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering Zilic, Leyla Garner, Philippa E Yu, Tong Roman, Sabiniano Haycock, John W Wilshaw, Stacy-Paul J Anat Original Articles Current nerve tissue engineering applications are adopting xenogeneic nerve tissue as potential nerve grafts to help aid nerve regeneration. However, there is little literature that describes the exact location, anatomy and physiology of these nerves to highlight their potential as a donor graft. The aim of this study was to identify and characterise the structural and extracellular matrix (ECM) components of porcine peripheral nerves in the hind leg. Methods included the dissection of porcine nerves, localisation, characterisation and quantification of the ECM components and identification of nerve cells. Results showed a noticeable variance between porcine and rat nerve (a commonly studied species) in terms of fascicle number. The study also revealed that when porcine peripheral nerves branch, a decrease in fascicle number and size was evident. Porcine ECM and nerve fascicles were found to be predominately comprised of collagen together with glycosaminoglycans, laminin and fibronectin. Immunolabelling for nerve growth factor receptor p75 also revealed the localisation of Schwann cells around and inside the fascicles. In conclusion, it is shown that porcine peripheral nerves possess a microstructure similar to that found in rat, and is not dissimilar to human. This finding could extend to the suggestion that due to the similarities in anatomy to human nerve, porcine nerves may have utility as a nerve graft providing guidance and support to regenerating axons. John Wiley & Sons, Ltd 2015-09 2015-07-21 /pmc/articles/PMC4560565/ /pubmed/26200940 http://dx.doi.org/10.1111/joa.12341 Text en © 2015 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Zilic, Leyla Garner, Philippa E Yu, Tong Roman, Sabiniano Haycock, John W Wilshaw, Stacy-Paul An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title | An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title_full | An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title_fullStr | An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title_full_unstemmed | An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title_short | An anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
title_sort | anatomical study of porcine peripheral nerve and its potential use in nerve tissue engineering |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560565/ https://www.ncbi.nlm.nih.gov/pubmed/26200940 http://dx.doi.org/10.1111/joa.12341 |
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