Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants

This data article refers to the report Δ(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technical...

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Detalles Bibliográficos
Autores principales: Lange, Kerstin, Poetsch, Ansgar, Schmid, Andreas, Julsing, Mattijs K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560726/
https://www.ncbi.nlm.nih.gov/pubmed/26401520
http://dx.doi.org/10.1016/j.dib.2015.07.033
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author Lange, Kerstin
Poetsch, Ansgar
Schmid, Andreas
Julsing, Mattijs K.
author_facet Lange, Kerstin
Poetsch, Ansgar
Schmid, Andreas
Julsing, Mattijs K.
author_sort Lange, Kerstin
collection PubMed
description This data article refers to the report Δ(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography.
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spelling pubmed-45607262015-09-23 Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants Lange, Kerstin Poetsch, Ansgar Schmid, Andreas Julsing, Mattijs K. Data Brief Data Article This data article refers to the report Δ(9)-Tetrahydrocannabinolic acid synthase (THCAS) production in Pichia pastoris enables chemical synthesis of cannabinoids (Lange et. al. 2015) [2]. THCAS was produced on a 2 L lab scale using recombinant P. pastoris KM71 KE1. Enrichment of THCAS as a technically pure enzyme was realized using dialysis and cationic exchange chromatography. nLC-ESI-MS/MS analysis identified THCAS in different fractions obtained by cationic exchange chromatography. Elsevier 2015-08-06 /pmc/articles/PMC4560726/ /pubmed/26401520 http://dx.doi.org/10.1016/j.dib.2015.07.033 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Lange, Kerstin
Poetsch, Ansgar
Schmid, Andreas
Julsing, Mattijs K.
Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title_full Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title_fullStr Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title_full_unstemmed Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title_short Enrichment and identification of Δ(9)-Tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
title_sort enrichment and identification of δ(9)-tetrahydrocannabinolic acid synthase from pichia pastoris culture supernatants
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560726/
https://www.ncbi.nlm.nih.gov/pubmed/26401520
http://dx.doi.org/10.1016/j.dib.2015.07.033
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