Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation

O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Lercher, Lukas, Raj, Ritu, Patel, Nisha A., Price, Joshua, Mohammed, Shabaz, Robinson, Carol V., Schofield, Christopher J., Davis, Benjamin G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560749/
https://www.ncbi.nlm.nih.gov/pubmed/26305776
http://dx.doi.org/10.1038/ncomms8978
_version_ 1782388950964371456
author Lercher, Lukas
Raj, Ritu
Patel, Nisha A.
Price, Joshua
Mohammed, Shabaz
Robinson, Carol V.
Schofield, Christopher J.
Davis, Benjamin G.
author_facet Lercher, Lukas
Raj, Ritu
Patel, Nisha A.
Price, Joshua
Mohammed, Shabaz
Robinson, Carol V.
Schofield, Christopher J.
Davis, Benjamin G.
author_sort Lercher, Lukas
collection PubMed
description O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an ‘open' chromatin state. The results suggest that a plausible molecular basis for one role of histone O-GlcNAcylation in epigenetic regulation is to lower the barrier for RNA polymerase passage and hence increase transcription.
format Online
Article
Text
id pubmed-4560749
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Nature Pub. Group
record_format MEDLINE/PubMed
spelling pubmed-45607492015-09-14 Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation Lercher, Lukas Raj, Ritu Patel, Nisha A. Price, Joshua Mohammed, Shabaz Robinson, Carol V. Schofield, Christopher J. Davis, Benjamin G. Nat Commun Article O-GlcNAcylation is a newly discovered histone modification implicated in transcriptional regulation, but no structural information on the physical effect of GlcNAcylation on chromatin exists. Here, we generate synthetic, pure GlcNAcylated histones and nucleosomes and reveal that GlcNAcylation can modulate structure through direct destabilization of H2A/H2B dimers in the nucleosome, thus promoting an ‘open' chromatin state. The results suggest that a plausible molecular basis for one role of histone O-GlcNAcylation in epigenetic regulation is to lower the barrier for RNA polymerase passage and hence increase transcription. Nature Pub. Group 2015-08-25 /pmc/articles/PMC4560749/ /pubmed/26305776 http://dx.doi.org/10.1038/ncomms8978 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lercher, Lukas
Raj, Ritu
Patel, Nisha A.
Price, Joshua
Mohammed, Shabaz
Robinson, Carol V.
Schofield, Christopher J.
Davis, Benjamin G.
Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title_full Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title_fullStr Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title_full_unstemmed Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title_short Generation of a synthetic GlcNAcylated nucleosome reveals regulation of stability by H2A-Thr101 GlcNAcylation
title_sort generation of a synthetic glcnacylated nucleosome reveals regulation of stability by h2a-thr101 glcnacylation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4560749/
https://www.ncbi.nlm.nih.gov/pubmed/26305776
http://dx.doi.org/10.1038/ncomms8978
work_keys_str_mv AT lercherlukas generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT rajritu generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT patelnishaa generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT pricejoshua generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT mohammedshabaz generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT robinsoncarolv generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT schofieldchristopherj generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation
AT davisbenjaming generationofasyntheticglcnacylatednucleosomerevealsregulationofstabilitybyh2athr101glcnacylation

Ejemplares similares