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Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia

Micro-RNAs (miRNAs) are regulators of gene expression that control various biological processes. The role of many identified miRNAs is not yet resolved. Recent evidence suggests that miRNA mutations and/or misexpression may contribute to genetic disorders. Point mutations in the seed region of MIR18...

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Autores principales: Abu-Amero, Khaled K., Helwa, Inas, Al-Muammar, Abdulrahman, Strickland, Shelby, Hauser, Michael A., Allingham, R. Rand, Liu, Yutao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561303/
https://www.ncbi.nlm.nih.gov/pubmed/26380287
http://dx.doi.org/10.1155/2015/604508
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author Abu-Amero, Khaled K.
Helwa, Inas
Al-Muammar, Abdulrahman
Strickland, Shelby
Hauser, Michael A.
Allingham, R. Rand
Liu, Yutao
author_facet Abu-Amero, Khaled K.
Helwa, Inas
Al-Muammar, Abdulrahman
Strickland, Shelby
Hauser, Michael A.
Allingham, R. Rand
Liu, Yutao
author_sort Abu-Amero, Khaled K.
collection PubMed
description Micro-RNAs (miRNAs) are regulators of gene expression that control various biological processes. The role of many identified miRNAs is not yet resolved. Recent evidence suggests that miRNA mutations and/or misexpression may contribute to genetic disorders. Point mutations in the seed region of MIR184 have been recently identified in Keratoconus (KC) patients with or without other corneal and lens abnormalities. We investigated mutations within MIR184 in KC patients from Saudi Arabia and examined the relative expression of miR-184 and miR-205 in human cornea. Ethnically matched KC cases (n = 134) were recruited and sequencing was performed using PCR-based Sanger sequencing and analyzed using the Sequencher 5.2 software. Expression of miR-184 and miR-205 was profiled in postmortem unaffected ocular tissues obtained from donors with no history of ocular diseases. miR-184 expression was 15-fold higher than that of miR-205 in cornea samples. No mutation(s) within the screened genomic region of MIR184 in KC cases was detected. This suggests that mutation in MIR184 is a rare cause of KC alone and may be more relevant to cases of KC associated with other ocular abnormalities. The increased expression of miR-184 versus miR-205 in normal cornea samples implies a possible role of miR184 in cornea development and/or corneal diseases.
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spelling pubmed-45613032015-09-14 Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia Abu-Amero, Khaled K. Helwa, Inas Al-Muammar, Abdulrahman Strickland, Shelby Hauser, Michael A. Allingham, R. Rand Liu, Yutao Biomed Res Int Research Article Micro-RNAs (miRNAs) are regulators of gene expression that control various biological processes. The role of many identified miRNAs is not yet resolved. Recent evidence suggests that miRNA mutations and/or misexpression may contribute to genetic disorders. Point mutations in the seed region of MIR184 have been recently identified in Keratoconus (KC) patients with or without other corneal and lens abnormalities. We investigated mutations within MIR184 in KC patients from Saudi Arabia and examined the relative expression of miR-184 and miR-205 in human cornea. Ethnically matched KC cases (n = 134) were recruited and sequencing was performed using PCR-based Sanger sequencing and analyzed using the Sequencher 5.2 software. Expression of miR-184 and miR-205 was profiled in postmortem unaffected ocular tissues obtained from donors with no history of ocular diseases. miR-184 expression was 15-fold higher than that of miR-205 in cornea samples. No mutation(s) within the screened genomic region of MIR184 in KC cases was detected. This suggests that mutation in MIR184 is a rare cause of KC alone and may be more relevant to cases of KC associated with other ocular abnormalities. The increased expression of miR-184 versus miR-205 in normal cornea samples implies a possible role of miR184 in cornea development and/or corneal diseases. Hindawi Publishing Corporation 2015 2015-08-24 /pmc/articles/PMC4561303/ /pubmed/26380287 http://dx.doi.org/10.1155/2015/604508 Text en Copyright © 2015 Khaled K. Abu-Amero et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Abu-Amero, Khaled K.
Helwa, Inas
Al-Muammar, Abdulrahman
Strickland, Shelby
Hauser, Michael A.
Allingham, R. Rand
Liu, Yutao
Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title_full Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title_fullStr Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title_full_unstemmed Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title_short Screening of the Seed Region of MIR184 in Keratoconus Patients from Saudi Arabia
title_sort screening of the seed region of mir184 in keratoconus patients from saudi arabia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4561303/
https://www.ncbi.nlm.nih.gov/pubmed/26380287
http://dx.doi.org/10.1155/2015/604508
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