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Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production

The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and...

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Autores principales: Lee, Ju Hee, Lim, Hun Jai, Lee, Chan Woo, Son, Kun-Ho, Son, Jong-Keun, Lee, Sang Kook, Kim, Hyun Pyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562339/
https://www.ncbi.nlm.nih.gov/pubmed/26379748
http://dx.doi.org/10.1155/2015/640846
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author Lee, Ju Hee
Lim, Hun Jai
Lee, Chan Woo
Son, Kun-Ho
Son, Jong-Keun
Lee, Sang Kook
Kim, Hyun Pyo
author_facet Lee, Ju Hee
Lim, Hun Jai
Lee, Chan Woo
Son, Kun-Ho
Son, Jong-Keun
Lee, Sang Kook
Kim, Hyun Pyo
author_sort Lee, Ju Hee
collection PubMed
description The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10–100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100–400 mg/kg and 30–60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders.
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spelling pubmed-45623392015-09-15 Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production Lee, Ju Hee Lim, Hun Jai Lee, Chan Woo Son, Kun-Ho Son, Jong-Keun Lee, Sang Kook Kim, Hyun Pyo Evid Based Complement Alternat Med Research Article The present study was designed to find pharmacologically active compound against airway inflammation from the roots of Asparagus cochinchinensis. The 70% ethanol extract of the roots of A. cochinchinensis (ACE) was found to inhibit IL-6 production from IL-1β-treated lung epithelial cells (A549) and the major constituent, methyl protodioscin (MP), also strongly inhibited the production of IL-6, IL-8, and tumor necrosis factor- (TNF-) α from A549 cells at 10–100 μM. This downregulating effect of proinflammatory cytokine production was found to be mediated, at least in part, via inhibition of c-Jun N-terminal kinase (JNK) and c-Jun activation pathway. When examined on an in vivo model of airway inflammation in mice, lipopolysaccharide- (LPS-) induced acute lung injury, ACE, and MP significantly inhibited cell infiltration in the bronchoalveolar lavage fluid by the oral treatment at doses of 100–400 mg/kg and 30–60 mg/kg, respectively. MP also inhibited the production of proinflammatory cytokines such as IL-6, TNF-α, and IL-1β in lung tissue. All of these findings provide scientific evidence supporting the role of A. cochinchinensis as a herbal remedy in treating airway inflammation and also suggest a therapeutic value of MP on airway inflammatory disorders. Hindawi Publishing Corporation 2015 2015-08-25 /pmc/articles/PMC4562339/ /pubmed/26379748 http://dx.doi.org/10.1155/2015/640846 Text en Copyright © 2015 Ju Hee Lee et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lee, Ju Hee
Lim, Hun Jai
Lee, Chan Woo
Son, Kun-Ho
Son, Jong-Keun
Lee, Sang Kook
Kim, Hyun Pyo
Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title_full Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title_fullStr Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title_full_unstemmed Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title_short Methyl Protodioscin from the Roots of Asparagus cochinchinensis Attenuates Airway Inflammation by Inhibiting Cytokine Production
title_sort methyl protodioscin from the roots of asparagus cochinchinensis attenuates airway inflammation by inhibiting cytokine production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562339/
https://www.ncbi.nlm.nih.gov/pubmed/26379748
http://dx.doi.org/10.1155/2015/640846
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