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VING: a software for visualization of deep sequencing signals

BACKGROUND: Next generation sequencing (NGS) data treatment often requires mapping sequenced reads onto a reference genome for further analysis. Mapped data are commonly visualized using genome browsers. However, such software are not suited for a publication-ready and versatile representation of NG...

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Autores principales: Descrimes, Marc, Zouari, Yousra Ben, Wery, Maxime, Legendre, Rachel, Gautheret, Daniel, Morillon, Antonin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562374/
https://www.ncbi.nlm.nih.gov/pubmed/26346985
http://dx.doi.org/10.1186/s13104-015-1404-5
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author Descrimes, Marc
Zouari, Yousra Ben
Wery, Maxime
Legendre, Rachel
Gautheret, Daniel
Morillon, Antonin
author_facet Descrimes, Marc
Zouari, Yousra Ben
Wery, Maxime
Legendre, Rachel
Gautheret, Daniel
Morillon, Antonin
author_sort Descrimes, Marc
collection PubMed
description BACKGROUND: Next generation sequencing (NGS) data treatment often requires mapping sequenced reads onto a reference genome for further analysis. Mapped data are commonly visualized using genome browsers. However, such software are not suited for a publication-ready and versatile representation of NGS data coverage, especially when multiple experiments are simultaneously treated. RESULTS: We developed ‘VING’, a stand-alone R script that takes as input NGS mapping files and genome annotations to produce accurate snapshots of the NGS coverage signal for any specified genomic region. VING offers multiple viewing options, including strand-specific views and a special heatmap mode for representing multiple experiments in a single figure. CONCLUSIONS: VING produces high-quality figures for NGS data representation in a genome region of interest. It is available at http://vm-gb.curie.fr/ving/. We also developed a Galaxy wrapper, available in the Galaxy tool shed with installation and usage instructions.
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spelling pubmed-45623742015-09-09 VING: a software for visualization of deep sequencing signals Descrimes, Marc Zouari, Yousra Ben Wery, Maxime Legendre, Rachel Gautheret, Daniel Morillon, Antonin BMC Res Notes Technical Note BACKGROUND: Next generation sequencing (NGS) data treatment often requires mapping sequenced reads onto a reference genome for further analysis. Mapped data are commonly visualized using genome browsers. However, such software are not suited for a publication-ready and versatile representation of NGS data coverage, especially when multiple experiments are simultaneously treated. RESULTS: We developed ‘VING’, a stand-alone R script that takes as input NGS mapping files and genome annotations to produce accurate snapshots of the NGS coverage signal for any specified genomic region. VING offers multiple viewing options, including strand-specific views and a special heatmap mode for representing multiple experiments in a single figure. CONCLUSIONS: VING produces high-quality figures for NGS data representation in a genome region of interest. It is available at http://vm-gb.curie.fr/ving/. We also developed a Galaxy wrapper, available in the Galaxy tool shed with installation and usage instructions. BioMed Central 2015-09-07 /pmc/articles/PMC4562374/ /pubmed/26346985 http://dx.doi.org/10.1186/s13104-015-1404-5 Text en © Descrimes et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Technical Note
Descrimes, Marc
Zouari, Yousra Ben
Wery, Maxime
Legendre, Rachel
Gautheret, Daniel
Morillon, Antonin
VING: a software for visualization of deep sequencing signals
title VING: a software for visualization of deep sequencing signals
title_full VING: a software for visualization of deep sequencing signals
title_fullStr VING: a software for visualization of deep sequencing signals
title_full_unstemmed VING: a software for visualization of deep sequencing signals
title_short VING: a software for visualization of deep sequencing signals
title_sort ving: a software for visualization of deep sequencing signals
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562374/
https://www.ncbi.nlm.nih.gov/pubmed/26346985
http://dx.doi.org/10.1186/s13104-015-1404-5
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