Cargando…
Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus)
Natural selection endows animals with the abilities to store lipid when food is abundant and to synthesize lipid when it is limited. However, the relevant adaptive strategy of lipid metabolism has not been clearly elucidated in fish. This study examined the systemic metabolic strategies of Nile tila...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562571/ https://www.ncbi.nlm.nih.gov/pubmed/26265749 http://dx.doi.org/10.14814/phy2.12485 |
_version_ | 1782389172326105088 |
---|---|
author | He, An-Yuan Ning, Li-Jun Chen, Li-Qiao Chen, Ya-Li Xing, Qi Li, Jia-Min Qiao, Fang Li, Dong-Liang Zhang, Mei-Ling Du, Zhen-Yu |
author_facet | He, An-Yuan Ning, Li-Jun Chen, Li-Qiao Chen, Ya-Li Xing, Qi Li, Jia-Min Qiao, Fang Li, Dong-Liang Zhang, Mei-Ling Du, Zhen-Yu |
author_sort | He, An-Yuan |
collection | PubMed |
description | Natural selection endows animals with the abilities to store lipid when food is abundant and to synthesize lipid when it is limited. However, the relevant adaptive strategy of lipid metabolism has not been clearly elucidated in fish. This study examined the systemic metabolic strategies of Nile tilapia to maintain lipid homeostasis when fed with low- or high-fat diets. Three diets with different lipid contents (1%, 7%, and 13%) were formulated and fed to tilapias for 10 weeks. At the end of the feeding trial, the growth rate, hepatic somatic index, and the triglyceride (TG) contents of serum, liver, muscle, and adipose tissue were comparable among three groups, whereas the total body lipid contents and the mass of adipose tissue increased with the increased dietary lipid levels. Overall quantitative PCR, western blotting and transcriptomic assays indicated that the liver was the primary responding organ to low-fat (LF) diet feeding, and the elevated glycolysis and accelerated biosynthesis of fatty acids (FA) in the liver is likely to be the main strategies of tilapia toward LF intake. In contrast, excess ingested lipid was preferentially stored in adipose tissue through increasing the capability of FA uptake and TG synthesis. Increasing numbers, but not enlarging size, of adipocytes may be the main strategy of Nile tilapia responding to continuous high-fat (HF) diet feeding. This is the first study illuminating the systemic adaptation of lipid metabolism responding to LF or HF diet in fish, and our results shed new light on fish physiology. |
format | Online Article Text |
id | pubmed-4562571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | John Wiley & Sons, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45625712015-09-14 Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) He, An-Yuan Ning, Li-Jun Chen, Li-Qiao Chen, Ya-Li Xing, Qi Li, Jia-Min Qiao, Fang Li, Dong-Liang Zhang, Mei-Ling Du, Zhen-Yu Physiol Rep Original Research Natural selection endows animals with the abilities to store lipid when food is abundant and to synthesize lipid when it is limited. However, the relevant adaptive strategy of lipid metabolism has not been clearly elucidated in fish. This study examined the systemic metabolic strategies of Nile tilapia to maintain lipid homeostasis when fed with low- or high-fat diets. Three diets with different lipid contents (1%, 7%, and 13%) were formulated and fed to tilapias for 10 weeks. At the end of the feeding trial, the growth rate, hepatic somatic index, and the triglyceride (TG) contents of serum, liver, muscle, and adipose tissue were comparable among three groups, whereas the total body lipid contents and the mass of adipose tissue increased with the increased dietary lipid levels. Overall quantitative PCR, western blotting and transcriptomic assays indicated that the liver was the primary responding organ to low-fat (LF) diet feeding, and the elevated glycolysis and accelerated biosynthesis of fatty acids (FA) in the liver is likely to be the main strategies of tilapia toward LF intake. In contrast, excess ingested lipid was preferentially stored in adipose tissue through increasing the capability of FA uptake and TG synthesis. Increasing numbers, but not enlarging size, of adipocytes may be the main strategy of Nile tilapia responding to continuous high-fat (HF) diet feeding. This is the first study illuminating the systemic adaptation of lipid metabolism responding to LF or HF diet in fish, and our results shed new light on fish physiology. John Wiley & Sons, Ltd 2015-08-11 /pmc/articles/PMC4562571/ /pubmed/26265749 http://dx.doi.org/10.14814/phy2.12485 Text en © 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society. http://creativecommons.org/licenses/by/4.0/ This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research He, An-Yuan Ning, Li-Jun Chen, Li-Qiao Chen, Ya-Li Xing, Qi Li, Jia-Min Qiao, Fang Li, Dong-Liang Zhang, Mei-Ling Du, Zhen-Yu Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title | Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title_full | Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title_fullStr | Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed | Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title_short | Systemic adaptation of lipid metabolism in response to low- and high-fat diet in Nile tilapia (Oreochromis niloticus) |
title_sort | systemic adaptation of lipid metabolism in response to low- and high-fat diet in nile tilapia (oreochromis niloticus) |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4562571/ https://www.ncbi.nlm.nih.gov/pubmed/26265749 http://dx.doi.org/10.14814/phy2.12485 |
work_keys_str_mv | AT heanyuan systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT ninglijun systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT chenliqiao systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT chenyali systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT xingqi systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT lijiamin systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT qiaofang systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT lidongliang systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT zhangmeiling systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus AT duzhenyu systemicadaptationoflipidmetabolisminresponsetolowandhighfatdietinniletilapiaoreochromisniloticus |